Plant phytoestrogens interfere with normal estrogen-regulated functions like steroid synthesis and gonad physiology and morphology. Much evidence has been obtained by using high dose treatments or in vitro exposure to phytoestrogens but little is known about low, dietary level concentrations of these compounds, particularly coumestrol. In order to explore the possible effects on gonads and serum progesterone of oral 10, 20 or 40 μg/Kg body weight dose coumestrol were administered to three experimental groups and compared to a vehicle-only control group (n=5 animals per group) for two weeks and a similar period for treatment recovery. After treatment, testes and blood were obtained and processed for testis and sperm morphology alterations, and steroid hormone evaluation, respectively. Coumestrol treatment induces a significant dose-dependent testis volume decrease and a decrease in 17β hydroxysteroid dehydrogenase activity causing a progesterone increase in response to phytoestrogen concentration. These alterations impair the normal sperm production with an increase in abnormal head and tail shapes. These data strongly suggest a deleterious effect of oral, low concentration phytoestrogen content in adult male diets.
Published in | Cell Biology (Volume 2, Issue 4) |
DOI | 10.11648/j.cb.20140204.12 |
Page(s) | 36-40 |
Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
Copyright |
Copyright © The Author(s), 2014. Published by Science Publishing Group |
Phytoestrogens, Coumestrol, Testis Alterations, Serum Progesterone, Mice
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APA Style
Hector Serrano, Guillermo Mora-Ramiro, Sheila Peña-Corona, Pablo León-Ortíz, Arturo Salame-Mendez, et al. (2014). Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice. Cell Biology, 2(4), 36-40. https://doi.org/10.11648/j.cb.20140204.12
ACS Style
Hector Serrano; Guillermo Mora-Ramiro; Sheila Peña-Corona; Pablo León-Ortíz; Arturo Salame-Mendez, et al. Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice. Cell Biol. 2014, 2(4), 36-40. doi: 10.11648/j.cb.20140204.12
AMA Style
Hector Serrano, Guillermo Mora-Ramiro, Sheila Peña-Corona, Pablo León-Ortíz, Arturo Salame-Mendez, et al. Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice. Cell Biol. 2014;2(4):36-40. doi: 10.11648/j.cb.20140204.12
@article{10.11648/j.cb.20140204.12, author = {Hector Serrano and Guillermo Mora-Ramiro and Sheila Peña-Corona and Pablo León-Ortíz and Arturo Salame-Mendez and Enrique Mendieta-Márques and José Luis Gómez-Olivares and María Dolores García-Suárez}, title = {Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice}, journal = {Cell Biology}, volume = {2}, number = {4}, pages = {36-40}, doi = {10.11648/j.cb.20140204.12}, url = {https://doi.org/10.11648/j.cb.20140204.12}, eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20140204.12}, abstract = {Plant phytoestrogens interfere with normal estrogen-regulated functions like steroid synthesis and gonad physiology and morphology. Much evidence has been obtained by using high dose treatments or in vitro exposure to phytoestrogens but little is known about low, dietary level concentrations of these compounds, particularly coumestrol. In order to explore the possible effects on gonads and serum progesterone of oral 10, 20 or 40 μg/Kg body weight dose coumestrol were administered to three experimental groups and compared to a vehicle-only control group (n=5 animals per group) for two weeks and a similar period for treatment recovery. After treatment, testes and blood were obtained and processed for testis and sperm morphology alterations, and steroid hormone evaluation, respectively. Coumestrol treatment induces a significant dose-dependent testis volume decrease and a decrease in 17β hydroxysteroid dehydrogenase activity causing a progesterone increase in response to phytoestrogen concentration. These alterations impair the normal sperm production with an increase in abnormal head and tail shapes. These data strongly suggest a deleterious effect of oral, low concentration phytoestrogen content in adult male diets.}, year = {2014} }
TY - JOUR T1 - Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice AU - Hector Serrano AU - Guillermo Mora-Ramiro AU - Sheila Peña-Corona AU - Pablo León-Ortíz AU - Arturo Salame-Mendez AU - Enrique Mendieta-Márques AU - José Luis Gómez-Olivares AU - María Dolores García-Suárez Y1 - 2014/09/30 PY - 2014 N1 - https://doi.org/10.11648/j.cb.20140204.12 DO - 10.11648/j.cb.20140204.12 T2 - Cell Biology JF - Cell Biology JO - Cell Biology SP - 36 EP - 40 PB - Science Publishing Group SN - 2330-0183 UR - https://doi.org/10.11648/j.cb.20140204.12 AB - Plant phytoestrogens interfere with normal estrogen-regulated functions like steroid synthesis and gonad physiology and morphology. Much evidence has been obtained by using high dose treatments or in vitro exposure to phytoestrogens but little is known about low, dietary level concentrations of these compounds, particularly coumestrol. In order to explore the possible effects on gonads and serum progesterone of oral 10, 20 or 40 μg/Kg body weight dose coumestrol were administered to three experimental groups and compared to a vehicle-only control group (n=5 animals per group) for two weeks and a similar period for treatment recovery. After treatment, testes and blood were obtained and processed for testis and sperm morphology alterations, and steroid hormone evaluation, respectively. Coumestrol treatment induces a significant dose-dependent testis volume decrease and a decrease in 17β hydroxysteroid dehydrogenase activity causing a progesterone increase in response to phytoestrogen concentration. These alterations impair the normal sperm production with an increase in abnormal head and tail shapes. These data strongly suggest a deleterious effect of oral, low concentration phytoestrogen content in adult male diets. VL - 2 IS - 4 ER -