Skin Nourishing Effects of Biofield Energy Healing Treatment Based Herbomineral Formulation

Shirley Theresa Holmlund; Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; Sambhu Charan Mondal; Snehasis Jana

doi:doi:10.11648/j.ejpm.20170502.11

Skin Nourishing Effects of Biofield Energy Healing Treatment Based Herbomineral Formulation

Shirley Theresa Holmlund, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Published in European Journal of Preventive Medicine (Volume 5, Issue 2)

Received: 31 March 2017 Accepted: 13 April 2017 Published: 3 May 2017

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Abstract

The aim of the present study was undertaken to evaluate the effects of a Consciousness Energy Healing (The Trivedi Effect^®) Treatment based test formulation on skin health. The test formulation and DMEM were separated into two parts. One part of each received the Consciousness Energy Healing Treatment by Shirley Theresa Holmlund and were termed as the Biofield Energy Treated samples, while the other parts were considered as the untreated test samples. MTT assay showed more than 70% cells were viable in all the tested concentrations in three cell lines and that the test formulation was safe and nontoxic. The percent cell proliferation using BrdU assay was significantly increased by 343.57%, 100.29%, and 132.6% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 8.75 µg/mL compared to the UT-DMEM + UT-Test formulation group. The collagen synthesis was significantly increased by 5.94% and 10.84% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL with respect to the untreated group. Elastin level was significantly (p≤0.001) increased by 15.95%, 58.31%, and 47.18% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively compared to the untreated group. The level of hyaluronic acid was significantly increased by 15.13% and 68.56% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL compared to the untreated group. The level of melanin was significantly reduced by 17.06%, 6.94%, and 13.07% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.063 µg/mL compared to untreated group. The effect of skin protection upon exposure with UV-B rays displayed some alteration of the percent cell viability in all the tested groups. The wound healing activity by scratch assay exhibited significant wound closure and cell migration in the test formulation and DMEM in HFF-1 cells compared to the untreated group. Altogether, the data suggests that the Consciousness Energy Healing Treated DMEM and test formulation exhibited better skin protective responses compared to the untreated DMEM and test formulation group with respect to the skin health parameters. Therefore, the Biofield Energy Treated test formulation could be developed as a potential cosmetic product against various skin problems viz. eczema, vitiligo, psoriasis, acne, hives, rosacea, ichthyosis, seborrheic dermatitis, erythema, contact dermatitis, skin aging, wrinkles, etc.

Published in	European Journal of Preventive Medicine (Volume 5, Issue 2)
DOI	10.11648/j.ejpm.20170502.11
Page(s)	23-32
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Consciousness Energy Healing, The Trivedi Effect^®, Skin Protection, HFF-1; B16-F10, HaCaT, Wound Healing, Extracellular Matrix

References

[1]	Helmstädter A, Staiger C (2014) Traditional use of medicinal agents: A valid source of evidence. Drug Discov Today 19: 4-7.
[2]	Fabricant DS, Farnsworth NR (2001) The value of plants used in traditional medicine for drug discovery. Environ Health Perspect 109: 69-75.
[3]	Gao XH, Zhang L, Wei H, Chen HD (2008) Efficacy and safety of innovative cosmeceuticals. Clin Dermatol 26: 367-374.
[4]	Chanchal D, Swarnlata S (2008) Novel approaches in herbal cosmetics. J Cosmet Dermatol 7: 89-95.
[5]	Baumann L (2007) Skin ageing and its treatment. J Pathol 211: 241-251.
[6]	Uitto J (1997) Understanding premature skin aging. N Engl J Med 337: 1463-1465.
[7]	Park K (2015) Role of micronutrients in skin health and function. Biomol Ther (Seoul) 23: 207-217.
[8]	McDaniel S, Goldman GD (2002) Consequences of using escharotic agents as primary treatment for nonmelanoma skin cancer. Arch Dermatol 138: 1593-1596.
[9]	Dziaman T, Huzarski T, Gackowski D, Rozalski R, Siomek A, Szpila A, Guz J, Lubinski J, Wasowicz W, Roszkowski K, Olinski R (2009) Selenium supplementation reduced oxidative DNA damage in adnexectomized BRCA1 mutations carriers. Cancer Epidemiol Biomarkers Prev 18: 2923-2928.
[10]	Spallholz JE (2001) Selenium and the prevention of cancer Part II: Mechanisms for the carcinostatic activitiy of Se compounds. The Bulletin of Selenium-Tellurium Development Association 2001: 12.
[11]	Combs GF, Jr., Clark LC, Turnbull BW (1997) Reduction of cancer mortality and incidence by selenium supplementation. Med Klin (Munich) 92: 42-45.
[12]	Combs GF, Jr., Clark LC, Turnbull BW (1997) Reduction of cancer risk with an oral supplement of selenium. Biomed Environ Sci 10: 227-234.
[13]	Institute of Medicine (IOM), (2001). Dietary Reference Intakes for Vitamin A, Vitamin K, Arsenic, Boron, Chromium, Copper, Iodine, Iron, Manganese, Molybdenum, Nickel, Silicon, Vanadium, and Zinc. Food and Nutrition Board. Washington, DC, USA (Ed.), National Academy Press, Washington, DC, pp. 420-441.
[14]	Kisker C, Schindelin H, Rees DC (1997) Molybdenum-cofactor-containing enzymes: Structure and mechanism. Annu Rev Biochem 66: 233-267.
[15]	Boyera N, Galey I, Bernard BA (1998) Effect of vitamin C and its derivatives on collagen synthesis and cross-linking by normal human fibroblasts. Int J Cosmet Sci 20: 151-158.
[16]	Sugiyama Y, Kawakishi S, Osawa T (1996) Involvement of the β-diketone moiety in the antioxidant mechanism of tetrahydrocurcuminoids. Biochem Pharmacol 52: 519-525.
[17]	Lai CS, Wu JC, Yu SF, Badmaev V, Nagabhushanam K, Ho CT, Pan MH (2011) Tetrahydrocurcumin is more effective than curcumin in preventing azoxymethane-induced colon carcinogenesis. Mol Nutr Food Res 55: 1819-1828.
[18]	Hashim P (2011) Centella asiatica in food and beverage applications and its potential antioxidant and neuroprotective effect. Int Food Res J 18: 1215-1222.
[19]	Hong SS, Kim JH, Shim CK (2005) Advance formulation and pharmacological activity of hydrogel of titrated extract of Centella asiatica. Arch Pharma Res 28: 502-508.
[20]	Shetty BS, Udupa AL, Somayaji SN (2006). Effect of Centella asiatica L. on normal and dexamethasone suppressed wound healing in Wistar Albino rats. Int J Low Extrem Wounds 5: 137-143.
[21]	Barnes PM, Powell-Griner E, McFann K, Nahin RL (2004) Complementary and alternative medicine use among adults: United States, 2002. Adv Data 343: 1-19.
[22]	Myers R (2003) The basics of chemistry. Greenwood Press, Westport, Connecticut.
[23]	Movaffaghi Z, Farsi M (2009) Biofield therapies: Biophysical basis and biological regulations. Complement Ther Clin Pract 15: 35-37.
[24]	Yount G, Patil S, Dave U, Alves-dos-Santos L, Gon K, Arauz R, and Rachlin K (2013) Evaluation of biofield treatment dose and distance in a model of cancer cell death. J Altern Complement Med 19: 124-127.
[25]	Garland SN, Valentine D, Desai K, Li S, Langer C, Evans T, Mao JJ (2013) Complementary and alternative medicine use and benefit finding among cancer patients. J Altern Complement Med 19: 876-881.
[26]	Trivedi MK, Tallapragada RM (2008) A transcendental to changing metal powder characteristics. Met Powder Rep 63: 22-28, 31.
[27]	Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O (2015) Studies of the atomic and crystalline characteristics of ceramic oxide nano powders after bio field treatment. Ind Eng Manage 4: 161.
[28]	Dabhade VV, Tallapragada RR, Trivedi MK (2009) Effect of external energy on atomic, crystalline and powder characteristics of antimony and bismuth powders. Bull Mater Sci 32: 471-479.
[29]	Sances F, Flora E, Patil S, Spence A, Shinde V (2013) Impact of biofield treatment on ginseng and organic blueberry yield. Agrivita J Agric Sci 35: 22-29.
[30]	Lenssen AW (2013) Biofield and fungicide seed treatment influences on soybean productivity, seed quality and weed community. Agricultural Journal 83: 138-143.
[31]	Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) Antimicrobial sensitivity pattern of Pseudomonas fluorescens after biofield treatment. J Infect Dis Ther 3: 222.
[32]	Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Phenotypic and biotypic characterization of Klebsiella oxytoca: An impact of biofield treatment. J Microb Biochem Technol 7: 203-206.
[33]	Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) An effect of biofield treatment on multidrug-resistant Burkholderia cepacia: A multihost pathogen. J Trop Dis 3: 167.
[34]	Patil SA, Nayak GB, Barve SS, Tembe RP, Khan RR (2012) Impact of biofield treatment on growth and anatomical characteristics of Pogostemon cablin (Benth.). Biotechnology 11: 154-162.
[35]	Nayak G, Altekar N (2015) Effect of biofield treatment on plant growth and adaptation. J Environ Health Sci 1: 1-9.
[36]	Biological evaluation of medical devices - Part 5: Tests for in vitro cytotoxicity (ISO 10993-5: 2009), I. S. EN ISO, 10993-5: 2009.
[37]	Junquiera LC, Junqueira LC, Brentani RR (1979) A simple and sensitive method for the quantitative estimation of collagen. Anal Biochem 94: 96-99.
[38]	Hahn MS, Kobler JB, Starcher BC, Zeitels SM, Langer R (2006) Quantitative and comparative studies of the vocal fold extracellular matrix. I: Elastic fibers and hyaluronic acid. Ann Otol Rhinol Laryngol 115: 156-164.
[39]	Zhang L, Yoshida T, Kuroiwa Y (1992) Stimulation of melanin synthesis of B16-F10 mouse melanoma cells by bufalin. Life Sci 51: 17-24.
[40]	Fronza M, Heinzmann B, Hamburger M, Laufer S, Merfort I (2009) Determination of the wound healing effect of Calendula extracts using the scratch assay with 3T3 fibroblasts. J Ethnopharmacol 126: 463-467.
[41]	Wen KC, Shih IC, Hu JC, Liao ST, Su TW, Chiang HM (2011) Inhibitory effects of Terminalia catappa on UV-B-induced photodamage in fibroblast cell line. Evid Based Complement Alternat Med 2011: 904532.
[42]	Rozario T, DeSimone DW (2010) The extracellular matrix in development and morphogenesis: A dynamic view. Dev Biol 341: 126-140.
[43]	Yadav K, Singhal N, Rishi V, Yadav H (2014) Cell proliferation assays. eLS. John Wiley & Sons Ltd., Chichester.
[44]	Jeffrey J (1995) Metalloproteinases and tissue turnover. Wounds 7: 13A-22A.
[45]	Albini A, Adelmann-Grill BC (1985) Collagenolytic cleavage products of collagen Type I as chemoattractants for human dermal fibroblasts. Eur J Cell Biol 36: 104-107.
[46]	Almine JF, Wise SG, Weiss AS (2012) Elastin signaling in wound repair. Birth Defects Res C Embryo Today 96: 248-257.
[47]	Yin L, Morita A, Tsuji T (2001) Skin aging induced by ultraviolet exposure and tobacco smoking: evidence from epidemiological and molecular studies. Photodermatol Photoimmunol Photomed 17: 178-183.
[48]	Castelo-Branco C, Figueras F, Martínez de Osaba MJ, Vanrell JA (1998) Facial wrinkling in postmenopausal women. Effects of smoking status and hormone replacement therapy. Maturitas 29: 75-86.
[49]	Youn CS, Kwon OS, Won CH, Hwang EJ, Park BJ, Eun HC, Chung JH (2003) Effect of pregnancy and menopause on facial wrinkling in women. Acta Derm Venereol 83: 419-424.
[50]	Contet-Audonneau JL, Jeanmaire C, Pauly G (1999) A histological study of human wrinkle structures: comparison between sun-exposed areas of the face, with or without wrinkles, and sun-protected areas. Br J Dermatol 140: 1038-1047.
[51]	Brincat M, Moniz CJ, Studd JW, Darby A, Magos A, Emburey G, Versi E. (1985) Long-term effects of the menopause and sex hormones on skin thickness. Br J Obstet Gynaecol 92: 256-259.
[52]	Frantz C, Stewart KM, Weaver VM (2010) The extracellular matrix at a glance. J Cell Sci 123: 4195-4200.

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APA Style

Shirley Theresa Holmlund, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. (2017). Skin Nourishing Effects of Biofield Energy Healing Treatment Based Herbomineral Formulation. European Journal of Preventive Medicine, 5(2), 23-32. https://doi.org/10.11648/j.ejpm.20170502.11

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ACS Style

Shirley Theresa Holmlund; Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak, et al. Skin Nourishing Effects of Biofield Energy Healing Treatment Based Herbomineral Formulation. Eur. J. Prev. Med. 2017, 5(2), 23-32. doi: 10.11648/j.ejpm.20170502.11

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AMA Style

Shirley Theresa Holmlund, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. Skin Nourishing Effects of Biofield Energy Healing Treatment Based Herbomineral Formulation. Eur J Prev Med. 2017;5(2):23-32. doi: 10.11648/j.ejpm.20170502.11

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@article{10.11648/j.ejpm.20170502.11,
  author = {Shirley Theresa Holmlund and Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and Sambhu Charan Mondal and Snehasis Jana},
  title = {Skin Nourishing Effects of Biofield Energy Healing Treatment Based Herbomineral Formulation},
  journal = {European Journal of Preventive Medicine},
  volume = {5},
  number = {2},
  pages = {23-32},
  doi = {10.11648/j.ejpm.20170502.11},
  url = {https://doi.org/10.11648/j.ejpm.20170502.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejpm.20170502.11},
  abstract = {The aim of the present study was undertaken to evaluate the effects of a Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation on skin health. The test formulation and DMEM were separated into two parts. One part of each received the Consciousness Energy Healing Treatment by Shirley Theresa Holmlund and were termed as the Biofield Energy Treated samples, while the other parts were considered as the untreated test samples. MTT assay showed more than 70% cells were viable in all the tested concentrations in three cell lines and that the test formulation was safe and nontoxic. The percent cell proliferation using BrdU assay was significantly increased by 343.57%, 100.29%, and 132.6% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 8.75 µg/mL compared to the UT-DMEM + UT-Test formulation group. The collagen synthesis was significantly increased by 5.94% and 10.84% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL with respect to the untreated group. Elastin level was significantly (p≤0.001) increased by 15.95%, 58.31%, and 47.18% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively compared to the untreated group. The level of hyaluronic acid was significantly increased by 15.13% and 68.56% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL compared to the untreated group. The level of melanin was significantly reduced by 17.06%, 6.94%, and 13.07% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.063 µg/mL compared to untreated group. The effect of skin protection upon exposure with UV-B rays displayed some alteration of the percent cell viability in all the tested groups. The wound healing activity by scratch assay exhibited significant wound closure and cell migration in the test formulation and DMEM in HFF-1 cells compared to the untreated group. Altogether, the data suggests that the Consciousness Energy Healing Treated DMEM and test formulation exhibited better skin protective responses compared to the untreated DMEM and test formulation group with respect to the skin health parameters. Therefore, the Biofield Energy Treated test formulation could be developed as a potential cosmetic product against various skin problems viz. eczema, vitiligo, psoriasis, acne, hives, rosacea, ichthyosis, seborrheic dermatitis, erythema, contact dermatitis, skin aging, wrinkles, etc.},
 year = {2017}
}

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TY  - JOUR
T1  - Skin Nourishing Effects of Biofield Energy Healing Treatment Based Herbomineral Formulation
AU  - Shirley Theresa Holmlund
AU  - Mahendra Kumar Trivedi
AU  - Alice Branton
AU  - Dahryn Trivedi
AU  - Gopal Nayak
AU  - Sambhu Charan Mondal
AU  - Snehasis Jana
Y1  - 2017/05/03
PY  - 2017
N1  - https://doi.org/10.11648/j.ejpm.20170502.11
DO  - 10.11648/j.ejpm.20170502.11
T2  - European Journal of Preventive Medicine
JF  - European Journal of Preventive Medicine
JO  - European Journal of Preventive Medicine
SP  - 23
EP  - 32
PB  - Science Publishing Group
SN  - 2330-8230
UR  - https://doi.org/10.11648/j.ejpm.20170502.11
AB  - The aim of the present study was undertaken to evaluate the effects of a Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation on skin health. The test formulation and DMEM were separated into two parts. One part of each received the Consciousness Energy Healing Treatment by Shirley Theresa Holmlund and were termed as the Biofield Energy Treated samples, while the other parts were considered as the untreated test samples. MTT assay showed more than 70% cells were viable in all the tested concentrations in three cell lines and that the test formulation was safe and nontoxic. The percent cell proliferation using BrdU assay was significantly increased by 343.57%, 100.29%, and 132.6% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 8.75 µg/mL compared to the UT-DMEM + UT-Test formulation group. The collagen synthesis was significantly increased by 5.94% and 10.84% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL with respect to the untreated group. Elastin level was significantly (p≤0.001) increased by 15.95%, 58.31%, and 47.18% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively compared to the untreated group. The level of hyaluronic acid was significantly increased by 15.13% and 68.56% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL compared to the untreated group. The level of melanin was significantly reduced by 17.06%, 6.94%, and 13.07% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.063 µg/mL compared to untreated group. The effect of skin protection upon exposure with UV-B rays displayed some alteration of the percent cell viability in all the tested groups. The wound healing activity by scratch assay exhibited significant wound closure and cell migration in the test formulation and DMEM in HFF-1 cells compared to the untreated group. Altogether, the data suggests that the Consciousness Energy Healing Treated DMEM and test formulation exhibited better skin protective responses compared to the untreated DMEM and test formulation group with respect to the skin health parameters. Therefore, the Biofield Energy Treated test formulation could be developed as a potential cosmetic product against various skin problems viz. eczema, vitiligo, psoriasis, acne, hives, rosacea, ichthyosis, seborrheic dermatitis, erythema, contact dermatitis, skin aging, wrinkles, etc.
VL  - 5
IS  - 2
ER  -

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Author Information

Shirley Theresa Holmlund

Trivedi Global, Inc., Henderson, Nevada, USA
Mahendra Kumar Trivedi

Trivedi Global, Inc., Henderson, Nevada, USA
Alice Branton

Trivedi Global, Inc., Henderson, Nevada, USA
Dahryn Trivedi

Trivedi Global, Inc., Henderson, Nevada, USA
Gopal Nayak

Trivedi Global, Inc., Henderson, Nevada, USA
Sambhu Charan Mondal

Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana

Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

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APA Style

Shirley Theresa Holmlund, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. (2017). Skin Nourishing Effects of Biofield Energy Healing Treatment Based Herbomineral Formulation. European Journal of Preventive Medicine, 5(2), 23-32. https://doi.org/10.11648/j.ejpm.20170502.11

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ACS Style

Shirley Theresa Holmlund; Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak, et al. Skin Nourishing Effects of Biofield Energy Healing Treatment Based Herbomineral Formulation. Eur. J. Prev. Med. 2017, 5(2), 23-32. doi: 10.11648/j.ejpm.20170502.11

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AMA Style

Shirley Theresa Holmlund, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. Skin Nourishing Effects of Biofield Energy Healing Treatment Based Herbomineral Formulation. Eur J Prev Med. 2017;5(2):23-32. doi: 10.11648/j.ejpm.20170502.11

Copy | Download

@article{10.11648/j.ejpm.20170502.11,
  author = {Shirley Theresa Holmlund and Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and Sambhu Charan Mondal and Snehasis Jana},
  title = {Skin Nourishing Effects of Biofield Energy Healing Treatment Based Herbomineral Formulation},
  journal = {European Journal of Preventive Medicine},
  volume = {5},
  number = {2},
  pages = {23-32},
  doi = {10.11648/j.ejpm.20170502.11},
  url = {https://doi.org/10.11648/j.ejpm.20170502.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejpm.20170502.11},
  abstract = {The aim of the present study was undertaken to evaluate the effects of a Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation on skin health. The test formulation and DMEM were separated into two parts. One part of each received the Consciousness Energy Healing Treatment by Shirley Theresa Holmlund and were termed as the Biofield Energy Treated samples, while the other parts were considered as the untreated test samples. MTT assay showed more than 70% cells were viable in all the tested concentrations in three cell lines and that the test formulation was safe and nontoxic. The percent cell proliferation using BrdU assay was significantly increased by 343.57%, 100.29%, and 132.6% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 8.75 µg/mL compared to the UT-DMEM + UT-Test formulation group. The collagen synthesis was significantly increased by 5.94% and 10.84% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL with respect to the untreated group. Elastin level was significantly (p≤0.001) increased by 15.95%, 58.31%, and 47.18% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively compared to the untreated group. The level of hyaluronic acid was significantly increased by 15.13% and 68.56% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL compared to the untreated group. The level of melanin was significantly reduced by 17.06%, 6.94%, and 13.07% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.063 µg/mL compared to untreated group. The effect of skin protection upon exposure with UV-B rays displayed some alteration of the percent cell viability in all the tested groups. The wound healing activity by scratch assay exhibited significant wound closure and cell migration in the test formulation and DMEM in HFF-1 cells compared to the untreated group. Altogether, the data suggests that the Consciousness Energy Healing Treated DMEM and test formulation exhibited better skin protective responses compared to the untreated DMEM and test formulation group with respect to the skin health parameters. Therefore, the Biofield Energy Treated test formulation could be developed as a potential cosmetic product against various skin problems viz. eczema, vitiligo, psoriasis, acne, hives, rosacea, ichthyosis, seborrheic dermatitis, erythema, contact dermatitis, skin aging, wrinkles, etc.},
 year = {2017}
}

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TY  - JOUR
T1  - Skin Nourishing Effects of Biofield Energy Healing Treatment Based Herbomineral Formulation
AU  - Shirley Theresa Holmlund
AU  - Mahendra Kumar Trivedi
AU  - Alice Branton
AU  - Dahryn Trivedi
AU  - Gopal Nayak
AU  - Sambhu Charan Mondal
AU  - Snehasis Jana
Y1  - 2017/05/03
PY  - 2017
N1  - https://doi.org/10.11648/j.ejpm.20170502.11
DO  - 10.11648/j.ejpm.20170502.11
T2  - European Journal of Preventive Medicine
JF  - European Journal of Preventive Medicine
JO  - European Journal of Preventive Medicine
SP  - 23
EP  - 32
PB  - Science Publishing Group
SN  - 2330-8230
UR  - https://doi.org/10.11648/j.ejpm.20170502.11
AB  - The aim of the present study was undertaken to evaluate the effects of a Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation on skin health. The test formulation and DMEM were separated into two parts. One part of each received the Consciousness Energy Healing Treatment by Shirley Theresa Holmlund and were termed as the Biofield Energy Treated samples, while the other parts were considered as the untreated test samples. MTT assay showed more than 70% cells were viable in all the tested concentrations in three cell lines and that the test formulation was safe and nontoxic. The percent cell proliferation using BrdU assay was significantly increased by 343.57%, 100.29%, and 132.6% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 8.75 µg/mL compared to the UT-DMEM + UT-Test formulation group. The collagen synthesis was significantly increased by 5.94% and 10.84% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL with respect to the untreated group. Elastin level was significantly (p≤0.001) increased by 15.95%, 58.31%, and 47.18% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively compared to the untreated group. The level of hyaluronic acid was significantly increased by 15.13% and 68.56% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL compared to the untreated group. The level of melanin was significantly reduced by 17.06%, 6.94%, and 13.07% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.063 µg/mL compared to untreated group. The effect of skin protection upon exposure with UV-B rays displayed some alteration of the percent cell viability in all the tested groups. The wound healing activity by scratch assay exhibited significant wound closure and cell migration in the test formulation and DMEM in HFF-1 cells compared to the untreated group. Altogether, the data suggests that the Consciousness Energy Healing Treated DMEM and test formulation exhibited better skin protective responses compared to the untreated DMEM and test formulation group with respect to the skin health parameters. Therefore, the Biofield Energy Treated test formulation could be developed as a potential cosmetic product against various skin problems viz. eczema, vitiligo, psoriasis, acne, hives, rosacea, ichthyosis, seborrheic dermatitis, erythema, contact dermatitis, skin aging, wrinkles, etc.
VL  - 5
IS  - 2
ER  -

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