Zidovudine is a drugs used in the management of Human Immunodeficiency Virus (HIV) and Acquired Immunodeficiency Syndrome (AIDS) infection in sub-Saharan Africa in combination with other drugs. The objective of this research was to investigate the potential harmful effects of this drug on the histology of the cerebellum of Wistar rats. Twenty male Wistar rats were used for this study. The rats were divided into 2 groups of 10 rats each. Group A served as the control and was administered with 1 ml of distill water, while group B was administered with 8.57mg/kg of zidovudine daily for 30 days, after which the rats where sacrificed and each cerebellum was harvested, processed and stained using haematoxylin and Eosin (H/E), silver impregnation method. Paraffin impregnated Glial Fibrilar Acidic Protein (GFAP), Neuron Specific Enolase (NSE) and Neurofilament (NF) immunochemistry methods. Stained slides were viewed using light microscope. Results showed that, the cerebellum of Groups B animals were affected with moderate to severe shrinking and distortion of the Purkinje cells and granular cells, when compared with the control. Group B animals, also showed more expression of GFAP, NSE and NF staining in their cerebellum than the control. This suggests that zidovudine is harmful to the cerebellum and should be taken with caution
Published in | American Journal of Bioscience and Bioengineering (Volume 3, Issue 3) |
DOI | 10.11648/j.bio.20150303.11 |
Page(s) | 17-21 |
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2015. Published by Science Publishing Group |
Zidovudine, Cerebellum, Human Immunodeficiency Virus
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APA Style
Aniekan Imo Peter, Gabriel Joseph Ekandem, Anozeng Oyono Igiri, Moses Bassey Ekong, Dianabasi King Udoh. (2015). Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine. American Journal of Bioscience and Bioengineering, 3(3), 17-21. https://doi.org/10.11648/j.bio.20150303.11
ACS Style
Aniekan Imo Peter; Gabriel Joseph Ekandem; Anozeng Oyono Igiri; Moses Bassey Ekong; Dianabasi King Udoh. Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine. Am. J. BioSci. Bioeng. 2015, 3(3), 17-21. doi: 10.11648/j.bio.20150303.11
AMA Style
Aniekan Imo Peter, Gabriel Joseph Ekandem, Anozeng Oyono Igiri, Moses Bassey Ekong, Dianabasi King Udoh. Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine. Am J BioSci Bioeng. 2015;3(3):17-21. doi: 10.11648/j.bio.20150303.11
@article{10.11648/j.bio.20150303.11, author = {Aniekan Imo Peter and Gabriel Joseph Ekandem and Anozeng Oyono Igiri and Moses Bassey Ekong and Dianabasi King Udoh}, title = {Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine}, journal = {American Journal of Bioscience and Bioengineering}, volume = {3}, number = {3}, pages = {17-21}, doi = {10.11648/j.bio.20150303.11}, url = {https://doi.org/10.11648/j.bio.20150303.11}, eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20150303.11}, abstract = {Zidovudine is a drugs used in the management of Human Immunodeficiency Virus (HIV) and Acquired Immunodeficiency Syndrome (AIDS) infection in sub-Saharan Africa in combination with other drugs. The objective of this research was to investigate the potential harmful effects of this drug on the histology of the cerebellum of Wistar rats. Twenty male Wistar rats were used for this study. The rats were divided into 2 groups of 10 rats each. Group A served as the control and was administered with 1 ml of distill water, while group B was administered with 8.57mg/kg of zidovudine daily for 30 days, after which the rats where sacrificed and each cerebellum was harvested, processed and stained using haematoxylin and Eosin (H/E), silver impregnation method. Paraffin impregnated Glial Fibrilar Acidic Protein (GFAP), Neuron Specific Enolase (NSE) and Neurofilament (NF) immunochemistry methods. Stained slides were viewed using light microscope. Results showed that, the cerebellum of Groups B animals were affected with moderate to severe shrinking and distortion of the Purkinje cells and granular cells, when compared with the control. Group B animals, also showed more expression of GFAP, NSE and NF staining in their cerebellum than the control. This suggests that zidovudine is harmful to the cerebellum and should be taken with caution}, year = {2015} }
TY - JOUR T1 - Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine AU - Aniekan Imo Peter AU - Gabriel Joseph Ekandem AU - Anozeng Oyono Igiri AU - Moses Bassey Ekong AU - Dianabasi King Udoh Y1 - 2015/08/19 PY - 2015 N1 - https://doi.org/10.11648/j.bio.20150303.11 DO - 10.11648/j.bio.20150303.11 T2 - American Journal of Bioscience and Bioengineering JF - American Journal of Bioscience and Bioengineering JO - American Journal of Bioscience and Bioengineering SP - 17 EP - 21 PB - Science Publishing Group SN - 2328-5893 UR - https://doi.org/10.11648/j.bio.20150303.11 AB - Zidovudine is a drugs used in the management of Human Immunodeficiency Virus (HIV) and Acquired Immunodeficiency Syndrome (AIDS) infection in sub-Saharan Africa in combination with other drugs. The objective of this research was to investigate the potential harmful effects of this drug on the histology of the cerebellum of Wistar rats. Twenty male Wistar rats were used for this study. The rats were divided into 2 groups of 10 rats each. Group A served as the control and was administered with 1 ml of distill water, while group B was administered with 8.57mg/kg of zidovudine daily for 30 days, after which the rats where sacrificed and each cerebellum was harvested, processed and stained using haematoxylin and Eosin (H/E), silver impregnation method. Paraffin impregnated Glial Fibrilar Acidic Protein (GFAP), Neuron Specific Enolase (NSE) and Neurofilament (NF) immunochemistry methods. Stained slides were viewed using light microscope. Results showed that, the cerebellum of Groups B animals were affected with moderate to severe shrinking and distortion of the Purkinje cells and granular cells, when compared with the control. Group B animals, also showed more expression of GFAP, NSE and NF staining in their cerebellum than the control. This suggests that zidovudine is harmful to the cerebellum and should be taken with caution VL - 3 IS - 3 ER -