Immunopharmacological Activity of Calcitriol Pre-Treated RAW264.7 Cell Line Stimulated by Phorbol 12-Myristate13-Acetate (PMA) and Formyl-Methionyl-Leucyl-Phenylalanine (FMLP) Agonist

Samson Ayodeji Olofinsae; Bartholomew Okechukwu Ibeh; Habu Josiah Bitrus; Jatinder Ahluwalia

doi:doi:10.11648/j.bio.20140204.11

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Immunopharmacological Activity of Calcitriol Pre-Treated RAW264.7 Cell Line Stimulated by Phorbol 12-Myristate13-Acetate (PMA) and Formyl-Methionyl-Leucyl-Phenylalanine (FMLP) Agonist

Samson Ayodeji Olofinsae, Bartholomew Okechukwu Ibeh, Habu Josiah Bitrus, Jatinder Ahluwalia

Published in American Journal of Bioscience and Bioengineering (Volume 2, Issue 4)

Received: 7 September 2014 Accepted: 15 September 2014 Published: 30 September 2014

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Abstract

The study investigates the effect of calcitriol treatment on oxygen consumption rate as generated by agonist stimulation of RAW 264.7 cell lines and its usefulness in eliciting reduced oxygen consumption in high respiratory burst-dependent disease state. Phorbol 12-myristate 13-acetate (PMA) and Formyl-methionyl-leucyl-phenylalanine (FMLP) were used to artificially stimulate oxygen consumption in cultured pro-monocytic RAW264.7 cell line. Samples of the cultured cells were previously prepared with calcitriol (1, 25- Dihydroxyvitamin D3) followed by a 72-hour incubation period. The percentage oxygen consumption was measured using the Clark oxygen electrode. There was a significant increase in oxygen consumption in FMLP treated cells (P<0.05) when compared with the PMA and the control groups. The PMA calcitriol-treated cells showed 24% oxygen consumption rate more than the control while FMLP treated cells was 57% higher. The result demonstrated that calcitriol, a known stimulant used to prep most cells for agonists stimulation of oxygen consumption may serve as a physiological moderator of oxygen consumption in immune cells when co-administered with agonist (PMA and/or FMLP). This may result to increased pathogen attack in a diseased state.

Published in	American Journal of Bioscience and Bioengineering (Volume 2, Issue 4)
DOI	10.11648/j.bio.20140204.11
Page(s)	55-59
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Respiratory Burst, Immunity, Oxidation, NADPH Oxidase, Promonocytes, RAW264.7, Calcitriol

References

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APA Style

Samson Ayodeji Olofinsae, Bartholomew Okechukwu Ibeh, Habu Josiah Bitrus, Jatinder Ahluwalia. (2014). Immunopharmacological Activity of Calcitriol Pre-Treated RAW264.7 Cell Line Stimulated by Phorbol 12-Myristate13-Acetate (PMA) and Formyl-Methionyl-Leucyl-Phenylalanine (FMLP) Agonist. American Journal of Bioscience and Bioengineering, 2(4), 55-59. https://doi.org/10.11648/j.bio.20140204.11

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ACS Style

Samson Ayodeji Olofinsae; Bartholomew Okechukwu Ibeh; Habu Josiah Bitrus; Jatinder Ahluwalia. Immunopharmacological Activity of Calcitriol Pre-Treated RAW264.7 Cell Line Stimulated by Phorbol 12-Myristate13-Acetate (PMA) and Formyl-Methionyl-Leucyl-Phenylalanine (FMLP) Agonist. Am. J. BioSci. Bioeng. 2014, 2(4), 55-59. doi: 10.11648/j.bio.20140204.11

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AMA Style

Samson Ayodeji Olofinsae, Bartholomew Okechukwu Ibeh, Habu Josiah Bitrus, Jatinder Ahluwalia. Immunopharmacological Activity of Calcitriol Pre-Treated RAW264.7 Cell Line Stimulated by Phorbol 12-Myristate13-Acetate (PMA) and Formyl-Methionyl-Leucyl-Phenylalanine (FMLP) Agonist. Am J BioSci Bioeng. 2014;2(4):55-59. doi: 10.11648/j.bio.20140204.11

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@article{10.11648/j.bio.20140204.11,
  author = {Samson Ayodeji Olofinsae and Bartholomew Okechukwu Ibeh and Habu Josiah Bitrus and Jatinder Ahluwalia},
  title = {Immunopharmacological Activity of Calcitriol Pre-Treated RAW264.7 Cell Line Stimulated by Phorbol 12-Myristate13-Acetate (PMA) and Formyl-Methionyl-Leucyl-Phenylalanine (FMLP) Agonist},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {2},
  number = {4},
  pages = {55-59},
  doi = {10.11648/j.bio.20140204.11},
  url = {https://doi.org/10.11648/j.bio.20140204.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20140204.11},
  abstract = {The study investigates the effect of calcitriol treatment on oxygen consumption rate as generated by agonist stimulation of RAW 264.7 cell lines and its usefulness in eliciting reduced oxygen consumption in high respiratory burst-dependent disease state. Phorbol 12-myristate 13-acetate (PMA) and Formyl-methionyl-leucyl-phenylalanine (FMLP) were used to artificially stimulate oxygen consumption in cultured pro-monocytic RAW264.7 cell line. Samples of the cultured cells were previously prepared with calcitriol (1, 25- Dihydroxyvitamin D3) followed by a 72-hour incubation period. The percentage oxygen consumption was measured using the Clark oxygen electrode. There was a significant increase in oxygen consumption in FMLP treated cells (P<0.05) when compared with the PMA and the control groups. The PMA calcitriol-treated cells showed 24% oxygen consumption rate more than the control while FMLP treated cells was 57% higher. The result demonstrated that calcitriol, a known stimulant used to prep most cells for agonists stimulation of oxygen consumption may serve as a physiological moderator of oxygen consumption in immune cells when co-administered with agonist (PMA and/or FMLP). This may result to increased pathogen attack in a diseased state.},
 year = {2014}
}

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TY  - JOUR
T1  - Immunopharmacological Activity of Calcitriol Pre-Treated RAW264.7 Cell Line Stimulated by Phorbol 12-Myristate13-Acetate (PMA) and Formyl-Methionyl-Leucyl-Phenylalanine (FMLP) Agonist
AU  - Samson Ayodeji Olofinsae
AU  - Bartholomew Okechukwu Ibeh
AU  - Habu Josiah Bitrus
AU  - Jatinder Ahluwalia
Y1  - 2014/09/30
PY  - 2014
N1  - https://doi.org/10.11648/j.bio.20140204.11
DO  - 10.11648/j.bio.20140204.11
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 55
EP  - 59
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20140204.11
AB  - The study investigates the effect of calcitriol treatment on oxygen consumption rate as generated by agonist stimulation of RAW 264.7 cell lines and its usefulness in eliciting reduced oxygen consumption in high respiratory burst-dependent disease state. Phorbol 12-myristate 13-acetate (PMA) and Formyl-methionyl-leucyl-phenylalanine (FMLP) were used to artificially stimulate oxygen consumption in cultured pro-monocytic RAW264.7 cell line. Samples of the cultured cells were previously prepared with calcitriol (1, 25- Dihydroxyvitamin D3) followed by a 72-hour incubation period. The percentage oxygen consumption was measured using the Clark oxygen electrode. There was a significant increase in oxygen consumption in FMLP treated cells (P<0.05) when compared with the PMA and the control groups. The PMA calcitriol-treated cells showed 24% oxygen consumption rate more than the control while FMLP treated cells was 57% higher. The result demonstrated that calcitriol, a known stimulant used to prep most cells for agonists stimulation of oxygen consumption may serve as a physiological moderator of oxygen consumption in immune cells when co-administered with agonist (PMA and/or FMLP). This may result to increased pathogen attack in a diseased state.
VL  - 2
IS  - 4
ER  -

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Author Information

Samson Ayodeji Olofinsae

School of Health, Sport and Bioscience, University of East London, United Kingdom
Bartholomew Okechukwu Ibeh

Departments of Medical Biotechnology, National Biotechnology Development Agency, Abuja, Nigeria
Habu Josiah Bitrus

Bioresources Development Center, Odi, National Biotechnology Development Agency, Nigeria
Jatinder Ahluwalia

School of Health, Sport and Bioscience, University of East London, United Kingdom

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APA Style

Samson Ayodeji Olofinsae, Bartholomew Okechukwu Ibeh, Habu Josiah Bitrus, Jatinder Ahluwalia. (2014). Immunopharmacological Activity of Calcitriol Pre-Treated RAW264.7 Cell Line Stimulated by Phorbol 12-Myristate13-Acetate (PMA) and Formyl-Methionyl-Leucyl-Phenylalanine (FMLP) Agonist. American Journal of Bioscience and Bioengineering, 2(4), 55-59. https://doi.org/10.11648/j.bio.20140204.11

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ACS Style

Samson Ayodeji Olofinsae; Bartholomew Okechukwu Ibeh; Habu Josiah Bitrus; Jatinder Ahluwalia. Immunopharmacological Activity of Calcitriol Pre-Treated RAW264.7 Cell Line Stimulated by Phorbol 12-Myristate13-Acetate (PMA) and Formyl-Methionyl-Leucyl-Phenylalanine (FMLP) Agonist. Am. J. BioSci. Bioeng. 2014, 2(4), 55-59. doi: 10.11648/j.bio.20140204.11

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AMA Style

Samson Ayodeji Olofinsae, Bartholomew Okechukwu Ibeh, Habu Josiah Bitrus, Jatinder Ahluwalia. Immunopharmacological Activity of Calcitriol Pre-Treated RAW264.7 Cell Line Stimulated by Phorbol 12-Myristate13-Acetate (PMA) and Formyl-Methionyl-Leucyl-Phenylalanine (FMLP) Agonist. Am J BioSci Bioeng. 2014;2(4):55-59. doi: 10.11648/j.bio.20140204.11

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@article{10.11648/j.bio.20140204.11,
  author = {Samson Ayodeji Olofinsae and Bartholomew Okechukwu Ibeh and Habu Josiah Bitrus and Jatinder Ahluwalia},
  title = {Immunopharmacological Activity of Calcitriol Pre-Treated RAW264.7 Cell Line Stimulated by Phorbol 12-Myristate13-Acetate (PMA) and Formyl-Methionyl-Leucyl-Phenylalanine (FMLP) Agonist},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {2},
  number = {4},
  pages = {55-59},
  doi = {10.11648/j.bio.20140204.11},
  url = {https://doi.org/10.11648/j.bio.20140204.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20140204.11},
  abstract = {The study investigates the effect of calcitriol treatment on oxygen consumption rate as generated by agonist stimulation of RAW 264.7 cell lines and its usefulness in eliciting reduced oxygen consumption in high respiratory burst-dependent disease state. Phorbol 12-myristate 13-acetate (PMA) and Formyl-methionyl-leucyl-phenylalanine (FMLP) were used to artificially stimulate oxygen consumption in cultured pro-monocytic RAW264.7 cell line. Samples of the cultured cells were previously prepared with calcitriol (1, 25- Dihydroxyvitamin D3) followed by a 72-hour incubation period. The percentage oxygen consumption was measured using the Clark oxygen electrode. There was a significant increase in oxygen consumption in FMLP treated cells (P<0.05) when compared with the PMA and the control groups. The PMA calcitriol-treated cells showed 24% oxygen consumption rate more than the control while FMLP treated cells was 57% higher. The result demonstrated that calcitriol, a known stimulant used to prep most cells for agonists stimulation of oxygen consumption may serve as a physiological moderator of oxygen consumption in immune cells when co-administered with agonist (PMA and/or FMLP). This may result to increased pathogen attack in a diseased state.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Immunopharmacological Activity of Calcitriol Pre-Treated RAW264.7 Cell Line Stimulated by Phorbol 12-Myristate13-Acetate (PMA) and Formyl-Methionyl-Leucyl-Phenylalanine (FMLP) Agonist
AU  - Samson Ayodeji Olofinsae
AU  - Bartholomew Okechukwu Ibeh
AU  - Habu Josiah Bitrus
AU  - Jatinder Ahluwalia
Y1  - 2014/09/30
PY  - 2014
N1  - https://doi.org/10.11648/j.bio.20140204.11
DO  - 10.11648/j.bio.20140204.11
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 55
EP  - 59
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20140204.11
AB  - The study investigates the effect of calcitriol treatment on oxygen consumption rate as generated by agonist stimulation of RAW 264.7 cell lines and its usefulness in eliciting reduced oxygen consumption in high respiratory burst-dependent disease state. Phorbol 12-myristate 13-acetate (PMA) and Formyl-methionyl-leucyl-phenylalanine (FMLP) were used to artificially stimulate oxygen consumption in cultured pro-monocytic RAW264.7 cell line. Samples of the cultured cells were previously prepared with calcitriol (1, 25- Dihydroxyvitamin D3) followed by a 72-hour incubation period. The percentage oxygen consumption was measured using the Clark oxygen electrode. There was a significant increase in oxygen consumption in FMLP treated cells (P<0.05) when compared with the PMA and the control groups. The PMA calcitriol-treated cells showed 24% oxygen consumption rate more than the control while FMLP treated cells was 57% higher. The result demonstrated that calcitriol, a known stimulant used to prep most cells for agonists stimulation of oxygen consumption may serve as a physiological moderator of oxygen consumption in immune cells when co-administered with agonist (PMA and/or FMLP). This may result to increased pathogen attack in a diseased state.
VL  - 2
IS  - 4
ER  -

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