The present investigation has been concentrated on the establishment of an efficacious method for in vitro regeneration of a Musa cultivar Gopi of Tripura. Focus has been greatly anchored to the sterilization procedure for assuring successful aseptic culture establishment of this cultivar. The culture protocol for shoot induction and proliferation comprised of four different treatments in basal MS medium fortified with 6-Benzylaminopurine of varying concentrations. The optimum response was encountered in the treatment of BAP in MS medium at concentration of 8mg l-1 for shoot bud induction and multiplication. Subsequent shoot growth and multiplication was achieved through repeated subcultures in media containing lower concentration (4 mg l-1). Root initiation was stimulated and brought about by treatments with two different concentrations of Indole butyric acid. The best rooting was manifested in treatment with IBA at concentration of 2mg l-1. The present study is an approach towards successful establishment of a simple and rapid clonal propagation of a potent Musa cultivar Gopi having traditional and commercial value from this region of Tripura, North-east India.
| Published in | American Journal of Plant Biology (Volume 3, Issue 1) |
| DOI | 10.11648/j.ajpb.20180301.13 |
| Page(s) | 12-16 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2018. Published by Science Publishing Group |
In Vitro Regeneration, Musa Cultivar Gopi, Benzylaminopurine, Indole Butyric Acid, Clonal Propagation, Tripura
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APA Style
Rabindra Kumar Sinha, Puja Rani Saha, Anath Bandhu Das, Satya Narayan Jena, Sangram Sinha. (2018). In Vitro Clonal Propagation of Musa Sp. Cultivar Gopi: A Palatable Banana of Tripura, India. American Journal of Plant Biology, 3(1), 12-16. https://doi.org/10.11648/j.ajpb.20180301.13
ACS Style
Rabindra Kumar Sinha; Puja Rani Saha; Anath Bandhu Das; Satya Narayan Jena; Sangram Sinha. In Vitro Clonal Propagation of Musa Sp. Cultivar Gopi: A Palatable Banana of Tripura, India. Am. J. Plant Biol. 2018, 3(1), 12-16. doi: 10.11648/j.ajpb.20180301.13
AMA Style
Rabindra Kumar Sinha, Puja Rani Saha, Anath Bandhu Das, Satya Narayan Jena, Sangram Sinha. In Vitro Clonal Propagation of Musa Sp. Cultivar Gopi: A Palatable Banana of Tripura, India. Am J Plant Biol. 2018;3(1):12-16. doi: 10.11648/j.ajpb.20180301.13
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Download@article{10.11648/j.ajpb.20180301.13,
author = {Rabindra Kumar Sinha and Puja Rani Saha and Anath Bandhu Das and Satya Narayan Jena and Sangram Sinha},
title = {In Vitro Clonal Propagation of Musa Sp. Cultivar Gopi: A Palatable Banana of Tripura, India},
journal = {American Journal of Plant Biology},
volume = {3},
number = {1},
pages = {12-16},
doi = {10.11648/j.ajpb.20180301.13},
url = {https://doi.org/10.11648/j.ajpb.20180301.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpb.20180301.13},
abstract = {The present investigation has been concentrated on the establishment of an efficacious method for in vitro regeneration of a Musa cultivar Gopi of Tripura. Focus has been greatly anchored to the sterilization procedure for assuring successful aseptic culture establishment of this cultivar. The culture protocol for shoot induction and proliferation comprised of four different treatments in basal MS medium fortified with 6-Benzylaminopurine of varying concentrations. The optimum response was encountered in the treatment of BAP in MS medium at concentration of 8mg l-1 for shoot bud induction and multiplication. Subsequent shoot growth and multiplication was achieved through repeated subcultures in media containing lower concentration (4 mg l-1). Root initiation was stimulated and brought about by treatments with two different concentrations of Indole butyric acid. The best rooting was manifested in treatment with IBA at concentration of 2mg l-1. The present study is an approach towards successful establishment of a simple and rapid clonal propagation of a potent Musa cultivar Gopi having traditional and commercial value from this region of Tripura, North-east India.},
year = {2018}
}
TY - JOUR T1 - In Vitro Clonal Propagation of Musa Sp. Cultivar Gopi: A Palatable Banana of Tripura, India AU - Rabindra Kumar Sinha AU - Puja Rani Saha AU - Anath Bandhu Das AU - Satya Narayan Jena AU - Sangram Sinha Y1 - 2018/05/30 PY - 2018 N1 - https://doi.org/10.11648/j.ajpb.20180301.13 DO - 10.11648/j.ajpb.20180301.13 T2 - American Journal of Plant Biology JF - American Journal of Plant Biology JO - American Journal of Plant Biology SP - 12 EP - 16 PB - Science Publishing Group SN - 2578-8337 UR - https://doi.org/10.11648/j.ajpb.20180301.13 AB - The present investigation has been concentrated on the establishment of an efficacious method for in vitro regeneration of a Musa cultivar Gopi of Tripura. Focus has been greatly anchored to the sterilization procedure for assuring successful aseptic culture establishment of this cultivar. The culture protocol for shoot induction and proliferation comprised of four different treatments in basal MS medium fortified with 6-Benzylaminopurine of varying concentrations. The optimum response was encountered in the treatment of BAP in MS medium at concentration of 8mg l-1 for shoot bud induction and multiplication. Subsequent shoot growth and multiplication was achieved through repeated subcultures in media containing lower concentration (4 mg l-1). Root initiation was stimulated and brought about by treatments with two different concentrations of Indole butyric acid. The best rooting was manifested in treatment with IBA at concentration of 2mg l-1. The present study is an approach towards successful establishment of a simple and rapid clonal propagation of a potent Musa cultivar Gopi having traditional and commercial value from this region of Tripura, North-east India. VL - 3 IS - 1 ER -
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