Genome-Wide Screen for Escherichia coli Genes Involved in Repressing Cell-To-Cell Transfer of a Nonconjugative pSC101-Derived Plasmid

Yuka Shibata; Akiko Matsumoto; Mutsumi Horino; Akiko Hirabayashi; Kozue Shirota; Chinatsu Kawano; Sumio Maeda

doi:doi:10.11648/j.ajls.20140206.13

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Genome-Wide Screen for Escherichia coli Genes Involved in Repressing Cell-To-Cell Transfer of a Nonconjugative pSC101-Derived Plasmid

Yuka Shibata, Akiko Matsumoto, Mutsumi Horino, Akiko Hirabayashi, Kozue Shirota, Chinatsu Kawano, Sumio Maeda

Published in American Journal of Life Sciences (Volume 2, Issue 6)

Received: 5 November 2014 Accepted: 18 November 2014 Published: 21 November 2014

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Abstract

Acquiring new genetic traits by lateral gene transfer is a bacterial strategy for environmental adaptations. We previously showed that Escherichia coli laterally transmits nonconjugative plasmids in cocultures that contain strains with or without the plasmid. Using a pMB1-derived plasmid and the Keio collection, a comprehensive library of E. coli knockout mutants for nonessential genes, we recently screened for genes responsible for promoting or repressing cell-to-cell plasmid transfer in recipient cells. In this study, we used a pSC101-derived plasmid, instead of a pMB1-derived plasmid, to screen for repressing genes and identified 29 “transfer-up” mutants. Among these, four mutants are common to those previously screened using a pMB1-derived plasmid. Although the roles of the 29 gene products in plasmid transfer mechanism remain uncertain, it is interesting that 28 of the 29 screened genes map to two limited regions on the E. coli chromosome: 18 genes at 34.25–35.31 min and 10 genes at 12.62–13.35 min. Because these two regions commonly contain termination (Ter) sites for DNA replication (TerC: 34.64 min and TerH: 12.91 min), it is possible that chromosomal mutations around specific Ter sites may affect plasmid acquisition in the recipient cells.

Published in	American Journal of Life Sciences (Volume 2, Issue 6)
DOI	10.11648/j.ajls.20140206.13
Page(s)	345-350
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Lateral Gene Transfer, Keio Collection, pSC101-Derived Plasmid, Ter Site, Escherichia coli

References

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Yuka Shibata, Akiko Matsumoto, Mutsumi Horino, Akiko Hirabayashi, Kozue Shirota, et al. (2014). Genome-Wide Screen for Escherichia coli Genes Involved in Repressing Cell-To-Cell Transfer of a Nonconjugative pSC101-Derived Plasmid. American Journal of Life Sciences, 2(6), 345-350. https://doi.org/10.11648/j.ajls.20140206.13

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ACS Style

Yuka Shibata; Akiko Matsumoto; Mutsumi Horino; Akiko Hirabayashi; Kozue Shirota, et al. Genome-Wide Screen for Escherichia coli Genes Involved in Repressing Cell-To-Cell Transfer of a Nonconjugative pSC101-Derived Plasmid. Am. J. Life Sci. 2014, 2(6), 345-350. doi: 10.11648/j.ajls.20140206.13

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AMA Style

Yuka Shibata, Akiko Matsumoto, Mutsumi Horino, Akiko Hirabayashi, Kozue Shirota, et al. Genome-Wide Screen for Escherichia coli Genes Involved in Repressing Cell-To-Cell Transfer of a Nonconjugative pSC101-Derived Plasmid. Am J Life Sci. 2014;2(6):345-350. doi: 10.11648/j.ajls.20140206.13

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@article{10.11648/j.ajls.20140206.13,
  author = {Yuka Shibata and Akiko Matsumoto and Mutsumi Horino and Akiko Hirabayashi and Kozue Shirota and Chinatsu Kawano and Sumio Maeda},
  title = {Genome-Wide Screen for Escherichia coli Genes Involved in Repressing Cell-To-Cell Transfer of a Nonconjugative pSC101-Derived Plasmid},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {6},
  pages = {345-350},
  doi = {10.11648/j.ajls.20140206.13},
  url = {https://doi.org/10.11648/j.ajls.20140206.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20140206.13},
  abstract = {Acquiring new genetic traits by lateral gene transfer is a bacterial strategy for environmental adaptations. We previously showed that Escherichia coli laterally transmits nonconjugative plasmids in cocultures that contain strains with or without the plasmid. Using a pMB1-derived plasmid and the Keio collection, a comprehensive library of E. coli knockout mutants for nonessential genes, we recently screened for genes responsible for promoting or repressing cell-to-cell plasmid transfer in recipient cells. In this study, we used a pSC101-derived plasmid, instead of a pMB1-derived plasmid, to screen for repressing genes and identified 29 “transfer-up” mutants. Among these, four mutants are common to those previously screened using a pMB1-derived plasmid. Although the roles of the 29 gene products in plasmid transfer mechanism remain uncertain, it is interesting that 28 of the 29 screened genes map to two limited regions on the E. coli chromosome: 18 genes at 34.25–35.31 min and 10 genes at 12.62–13.35 min. Because these two regions commonly contain termination (Ter) sites for DNA replication (TerC: 34.64 min and TerH: 12.91 min), it is possible that chromosomal mutations around specific Ter sites may affect plasmid acquisition in the recipient cells.},
 year = {2014}
}

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TY  - JOUR
T1  - Genome-Wide Screen for Escherichia coli Genes Involved in Repressing Cell-To-Cell Transfer of a Nonconjugative pSC101-Derived Plasmid
AU  - Yuka Shibata
AU  - Akiko Matsumoto
AU  - Mutsumi Horino
AU  - Akiko Hirabayashi
AU  - Kozue Shirota
AU  - Chinatsu Kawano
AU  - Sumio Maeda
Y1  - 2014/11/21
PY  - 2014
N1  - https://doi.org/10.11648/j.ajls.20140206.13
DO  - 10.11648/j.ajls.20140206.13
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 345
EP  - 350
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20140206.13
AB  - Acquiring new genetic traits by lateral gene transfer is a bacterial strategy for environmental adaptations. We previously showed that Escherichia coli laterally transmits nonconjugative plasmids in cocultures that contain strains with or without the plasmid. Using a pMB1-derived plasmid and the Keio collection, a comprehensive library of E. coli knockout mutants for nonessential genes, we recently screened for genes responsible for promoting or repressing cell-to-cell plasmid transfer in recipient cells. In this study, we used a pSC101-derived plasmid, instead of a pMB1-derived plasmid, to screen for repressing genes and identified 29 “transfer-up” mutants. Among these, four mutants are common to those previously screened using a pMB1-derived plasmid. Although the roles of the 29 gene products in plasmid transfer mechanism remain uncertain, it is interesting that 28 of the 29 screened genes map to two limited regions on the E. coli chromosome: 18 genes at 34.25–35.31 min and 10 genes at 12.62–13.35 min. Because these two regions commonly contain termination (Ter) sites for DNA replication (TerC: 34.64 min and TerH: 12.91 min), it is possible that chromosomal mutations around specific Ter sites may affect plasmid acquisition in the recipient cells.
VL  - 2
IS  - 6
ER  -

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Author Information

Yuka Shibata

Graduate School of Humanities and Sciences, Nara Women's University, Nara, Japan
Akiko Matsumoto

Graduate School of Humanities and Sciences, Nara Women's University, Nara, Japan
Mutsumi Horino

Faculty of Human Life and Environment, Nara Women's University, Nara, Japan
Akiko Hirabayashi

Faculty of Human Life and Environment, Nara Women's University, Nara, Japan
Kozue Shirota

Faculty of Human Life and Environment, Nara Women's University, Nara, Japan
Chinatsu Kawano

Faculty of Human Life and Environment, Nara Women's University, Nara, Japan
Sumio Maeda

Graduate School of Humanities and Sciences, Nara Women's University, Nara, Japan

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Yuka Shibata, Akiko Matsumoto, Mutsumi Horino, Akiko Hirabayashi, Kozue Shirota, et al. (2014). Genome-Wide Screen for Escherichia coli Genes Involved in Repressing Cell-To-Cell Transfer of a Nonconjugative pSC101-Derived Plasmid. American Journal of Life Sciences, 2(6), 345-350. https://doi.org/10.11648/j.ajls.20140206.13

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ACS Style

Yuka Shibata; Akiko Matsumoto; Mutsumi Horino; Akiko Hirabayashi; Kozue Shirota, et al. Genome-Wide Screen for Escherichia coli Genes Involved in Repressing Cell-To-Cell Transfer of a Nonconjugative pSC101-Derived Plasmid. Am. J. Life Sci. 2014, 2(6), 345-350. doi: 10.11648/j.ajls.20140206.13

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AMA Style

Yuka Shibata, Akiko Matsumoto, Mutsumi Horino, Akiko Hirabayashi, Kozue Shirota, et al. Genome-Wide Screen for Escherichia coli Genes Involved in Repressing Cell-To-Cell Transfer of a Nonconjugative pSC101-Derived Plasmid. Am J Life Sci. 2014;2(6):345-350. doi: 10.11648/j.ajls.20140206.13

Copy | Download

@article{10.11648/j.ajls.20140206.13,
  author = {Yuka Shibata and Akiko Matsumoto and Mutsumi Horino and Akiko Hirabayashi and Kozue Shirota and Chinatsu Kawano and Sumio Maeda},
  title = {Genome-Wide Screen for Escherichia coli Genes Involved in Repressing Cell-To-Cell Transfer of a Nonconjugative pSC101-Derived Plasmid},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {6},
  pages = {345-350},
  doi = {10.11648/j.ajls.20140206.13},
  url = {https://doi.org/10.11648/j.ajls.20140206.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20140206.13},
  abstract = {Acquiring new genetic traits by lateral gene transfer is a bacterial strategy for environmental adaptations. We previously showed that Escherichia coli laterally transmits nonconjugative plasmids in cocultures that contain strains with or without the plasmid. Using a pMB1-derived plasmid and the Keio collection, a comprehensive library of E. coli knockout mutants for nonessential genes, we recently screened for genes responsible for promoting or repressing cell-to-cell plasmid transfer in recipient cells. In this study, we used a pSC101-derived plasmid, instead of a pMB1-derived plasmid, to screen for repressing genes and identified 29 “transfer-up” mutants. Among these, four mutants are common to those previously screened using a pMB1-derived plasmid. Although the roles of the 29 gene products in plasmid transfer mechanism remain uncertain, it is interesting that 28 of the 29 screened genes map to two limited regions on the E. coli chromosome: 18 genes at 34.25–35.31 min and 10 genes at 12.62–13.35 min. Because these two regions commonly contain termination (Ter) sites for DNA replication (TerC: 34.64 min and TerH: 12.91 min), it is possible that chromosomal mutations around specific Ter sites may affect plasmid acquisition in the recipient cells.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Genome-Wide Screen for Escherichia coli Genes Involved in Repressing Cell-To-Cell Transfer of a Nonconjugative pSC101-Derived Plasmid
AU  - Yuka Shibata
AU  - Akiko Matsumoto
AU  - Mutsumi Horino
AU  - Akiko Hirabayashi
AU  - Kozue Shirota
AU  - Chinatsu Kawano
AU  - Sumio Maeda
Y1  - 2014/11/21
PY  - 2014
N1  - https://doi.org/10.11648/j.ajls.20140206.13
DO  - 10.11648/j.ajls.20140206.13
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 345
EP  - 350
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20140206.13
AB  - Acquiring new genetic traits by lateral gene transfer is a bacterial strategy for environmental adaptations. We previously showed that Escherichia coli laterally transmits nonconjugative plasmids in cocultures that contain strains with or without the plasmid. Using a pMB1-derived plasmid and the Keio collection, a comprehensive library of E. coli knockout mutants for nonessential genes, we recently screened for genes responsible for promoting or repressing cell-to-cell plasmid transfer in recipient cells. In this study, we used a pSC101-derived plasmid, instead of a pMB1-derived plasmid, to screen for repressing genes and identified 29 “transfer-up” mutants. Among these, four mutants are common to those previously screened using a pMB1-derived plasmid. Although the roles of the 29 gene products in plasmid transfer mechanism remain uncertain, it is interesting that 28 of the 29 screened genes map to two limited regions on the E. coli chromosome: 18 genes at 34.25–35.31 min and 10 genes at 12.62–13.35 min. Because these two regions commonly contain termination (Ter) sites for DNA replication (TerC: 34.64 min and TerH: 12.91 min), it is possible that chromosomal mutations around specific Ter sites may affect plasmid acquisition in the recipient cells.
VL  - 2
IS  - 6
ER  -

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