A Comparative Study of Antioxidant Activity, Total Phenolic and Flavonoid Contents in Different Parts of Helicteres isora L.

Amita Jain; Rashmi Ranade; Prem Pritam; Neelu Joshi; Sirisha Lakshmi Vavilala; Ankita Jain

doi:doi:10.11648/j.ajls.20140205.17

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A Comparative Study of Antioxidant Activity, Total Phenolic and Flavonoid Contents in Different Parts of Helicteres isora L.

Amita Jain, Rashmi Ranade, Prem Pritam, Neelu Joshi, Sirisha Lakshmi Vavilala, Ankita Jain

Published in American Journal of Life Sciences (Volume 2, Issue 5)

Received: 16 September 2014 Accepted: 20 October 2014 Published: 30 October 2014

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Abstract

Helicteres isora L., commonly known as Indian Screw Tree is a highly valued medicinal plant in South-East Asia. The various phytochemicals like phenols, flavonoids and other antioxidants that impart the medicinal properties in this plant, vary in their composition and concentration in different plant parts. In the present research, the total phenolic content, total flavonoids content and free radical scavenging activity (FRAP and DPPH assay) in fresh and dry sample extracts of leaf, bark, fruit and root of H. isora L., prepared in four different solvents (distilled water, ethanol, methanol and acetone) were studied, and their results compared using Pearson’s Correlation. The plant extracts were also subjected to RP-HPLC for detection and quantitation of naturally occurring phenolic compounds using six phenolic standards (Gallic acid, Vanillin, Catechol, Ferrulic acid, p-coumaric acid and Caffeic acid). The highest total phenolic content (7.22 mg/g GAE) and FRAP value (64.98 mg/g TE) were observed in aqueous dry root extract. The acetone extract of fresh leaf (57.08 mg/g of RE) was found richest in total flavonoids, while the methanolic extract of fresh fruit uniquely exhibited strong free radical scavenging activity as evidenced by the low IC50 value (34.37 mg/ml) in DPPH assay. The RP-HPLC analysis revealed that Catechol and Gallic acid were most abundantly found phenolic compounds in extracts of H. isora L. The total phenolic content showed strong positive correlation with free radical scavenging activity (FRAP and DPPH assays) in both fresh and dry plant parts, suggesting that phenols are the main compounds responsible for the antioxidant activity. The root of H. isora L. was found rich in phenolics and antioxidant capacity indicating its strong potential for medicinal use, followed by fruit, leaf and bark.

Published in	American Journal of Life Sciences (Volume 2, Issue 5)
DOI	10.11648/j.ajls.20140205.17
Page(s)	292-302
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Helicteres Isora L., Phenols, Flavonoids, DPPH, FRAP, RP-HPLC

References

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Amita Jain, Rashmi Ranade, Prem Pritam, Neelu Joshi, Sirisha Lakshmi Vavilala, et al. (2014). A Comparative Study of Antioxidant Activity, Total Phenolic and Flavonoid Contents in Different Parts of Helicteres isora L.. American Journal of Life Sciences, 2(5), 292-302. https://doi.org/10.11648/j.ajls.20140205.17

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ACS Style

Amita Jain; Rashmi Ranade; Prem Pritam; Neelu Joshi; Sirisha Lakshmi Vavilala, et al. A Comparative Study of Antioxidant Activity, Total Phenolic and Flavonoid Contents in Different Parts of Helicteres isora L.. Am. J. Life Sci. 2014, 2(5), 292-302. doi: 10.11648/j.ajls.20140205.17

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AMA Style

Amita Jain, Rashmi Ranade, Prem Pritam, Neelu Joshi, Sirisha Lakshmi Vavilala, et al. A Comparative Study of Antioxidant Activity, Total Phenolic and Flavonoid Contents in Different Parts of Helicteres isora L.. Am J Life Sci. 2014;2(5):292-302. doi: 10.11648/j.ajls.20140205.17

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@article{10.11648/j.ajls.20140205.17,
  author = {Amita Jain and Rashmi Ranade and Prem Pritam and Neelu Joshi and Sirisha Lakshmi Vavilala and Ankita Jain},
  title = {A Comparative Study of Antioxidant Activity, Total Phenolic and Flavonoid Contents in Different Parts of Helicteres isora L.},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {5},
  pages = {292-302},
  doi = {10.11648/j.ajls.20140205.17},
  url = {https://doi.org/10.11648/j.ajls.20140205.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20140205.17},
  abstract = {Helicteres isora L., commonly known as Indian Screw Tree is a highly valued medicinal plant in South-East Asia. The various phytochemicals like phenols, flavonoids and other antioxidants that impart the medicinal properties in this plant, vary in their composition and concentration in different plant parts. In the present research, the total phenolic content, total flavonoids content and free radical scavenging activity (FRAP and DPPH assay) in fresh and dry sample extracts of leaf, bark, fruit and root of H. isora L., prepared in four different solvents (distilled water, ethanol, methanol and acetone) were studied, and their results compared using Pearson’s Correlation. The plant extracts were also subjected to RP-HPLC for detection and quantitation of naturally occurring phenolic compounds using six phenolic standards (Gallic acid, Vanillin, Catechol, Ferrulic acid, p-coumaric acid and Caffeic acid). The highest total phenolic content (7.22 mg/g GAE) and FRAP value (64.98 mg/g TE) were observed in aqueous dry root extract. The acetone extract of fresh leaf (57.08 mg/g of RE) was found richest in total flavonoids, while the methanolic extract of fresh fruit uniquely exhibited strong free radical scavenging activity as evidenced by the low IC50 value (34.37 mg/ml) in DPPH assay. The RP-HPLC analysis revealed that Catechol and Gallic acid were most abundantly found phenolic compounds in extracts of H. isora L. The total phenolic content showed strong positive correlation with free radical scavenging activity (FRAP and DPPH assays) in both fresh and dry plant parts, suggesting that phenols are the main compounds responsible for the antioxidant activity. The root of H. isora L. was found rich in phenolics and antioxidant capacity indicating its strong potential for medicinal use, followed by fruit, leaf and bark.},
 year = {2014}
}

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TY - JOUR
T1 - A Comparative Study of Antioxidant Activity, Total Phenolic and Flavonoid Contents in Different Parts of Helicteres isora L.
AU - Amita Jain
AU - Rashmi Ranade
AU - Prem Pritam
AU - Neelu Joshi
AU - Sirisha Lakshmi Vavilala
AU - Ankita Jain
Y1 - 2014/10/30
PY - 2014
N1 - https://doi.org/10.11648/j.ajls.20140205.17
DO - 10.11648/j.ajls.20140205.17
T2 - American Journal of Life Sciences
JF - American Journal of Life Sciences
JO - American Journal of Life Sciences
SP - 292
EP - 302
PB - Science Publishing Group
SN - 2328-5737
UR - https://doi.org/10.11648/j.ajls.20140205.17
AB - Helicteres isora L., commonly known as Indian Screw Tree is a highly valued medicinal plant in South-East Asia. The various phytochemicals like phenols, flavonoids and other antioxidants that impart the medicinal properties in this plant, vary in their composition and concentration in different plant parts. In the present research, the total phenolic content, total flavonoids content and free radical scavenging activity (FRAP and DPPH assay) in fresh and dry sample extracts of leaf, bark, fruit and root of H. isora L., prepared in four different solvents (distilled water, ethanol, methanol and acetone) were studied, and their results compared using Pearson’s Correlation. The plant extracts were also subjected to RP-HPLC for detection and quantitation of naturally occurring phenolic compounds using six phenolic standards (Gallic acid, Vanillin, Catechol, Ferrulic acid, p-coumaric acid and Caffeic acid). The highest total phenolic content (7.22 mg/g GAE) and FRAP value (64.98 mg/g TE) were observed in aqueous dry root extract. The acetone extract of fresh leaf (57.08 mg/g of RE) was found richest in total flavonoids, while the methanolic extract of fresh fruit uniquely exhibited strong free radical scavenging activity as evidenced by the low IC50 value (34.37 mg/ml) in DPPH assay. The RP-HPLC analysis revealed that Catechol and Gallic acid were most abundantly found phenolic compounds in extracts of H. isora L. The total phenolic content showed strong positive correlation with free radical scavenging activity (FRAP and DPPH assays) in both fresh and dry plant parts, suggesting that phenols are the main compounds responsible for the antioxidant activity. The root of H. isora L. was found rich in phenolics and antioxidant capacity indicating its strong potential for medicinal use, followed by fruit, leaf and bark.
VL - 2
IS - 5
ER -

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Author Information

Amita Jain

School of Biotechnology and Bioinformatics, D.Y. Patil University, Sector No-15, CBD, Belapur, Navi Mumbai, India
Rashmi Ranade

School of Biotechnology and Bioinformatics, D.Y. Patil University, Sector No-15, CBD, Belapur, Navi Mumbai, India
Prem Pritam

School of Biotechnology and Bioinformatics, D.Y. Patil University, Sector No-15, CBD, Belapur, Navi Mumbai, India
Neelu Joshi

School of Biotechnology and Bioinformatics, D.Y. Patil University, Sector No-15, CBD, Belapur, Navi Mumbai, India
Sirisha Lakshmi Vavilala

School of Biotechnology and Bioinformatics, D.Y. Patil University, Sector No-15, CBD, Belapur, Navi Mumbai, India
Ankita Jain

School of Biotechnology and Bioinformatics, D.Y. Patil University, Sector No-15, CBD, Belapur, Navi Mumbai, India

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Amita Jain, Rashmi Ranade, Prem Pritam, Neelu Joshi, Sirisha Lakshmi Vavilala, et al. (2014). A Comparative Study of Antioxidant Activity, Total Phenolic and Flavonoid Contents in Different Parts of Helicteres isora L.. American Journal of Life Sciences, 2(5), 292-302. https://doi.org/10.11648/j.ajls.20140205.17

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Amita Jain; Rashmi Ranade; Prem Pritam; Neelu Joshi; Sirisha Lakshmi Vavilala, et al. A Comparative Study of Antioxidant Activity, Total Phenolic and Flavonoid Contents in Different Parts of Helicteres isora L.. Am. J. Life Sci. 2014, 2(5), 292-302. doi: 10.11648/j.ajls.20140205.17

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AMA Style

Amita Jain, Rashmi Ranade, Prem Pritam, Neelu Joshi, Sirisha Lakshmi Vavilala, et al. A Comparative Study of Antioxidant Activity, Total Phenolic and Flavonoid Contents in Different Parts of Helicteres isora L.. Am J Life Sci. 2014;2(5):292-302. doi: 10.11648/j.ajls.20140205.17

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@article{10.11648/j.ajls.20140205.17,
  author = {Amita Jain and Rashmi Ranade and Prem Pritam and Neelu Joshi and Sirisha Lakshmi Vavilala and Ankita Jain},
  title = {A Comparative Study of Antioxidant Activity, Total Phenolic and Flavonoid Contents in Different Parts of Helicteres isora L.},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {5},
  pages = {292-302},
  doi = {10.11648/j.ajls.20140205.17},
  url = {https://doi.org/10.11648/j.ajls.20140205.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20140205.17},
  abstract = {Helicteres isora L., commonly known as Indian Screw Tree is a highly valued medicinal plant in South-East Asia. The various phytochemicals like phenols, flavonoids and other antioxidants that impart the medicinal properties in this plant, vary in their composition and concentration in different plant parts. In the present research, the total phenolic content, total flavonoids content and free radical scavenging activity (FRAP and DPPH assay) in fresh and dry sample extracts of leaf, bark, fruit and root of H. isora L., prepared in four different solvents (distilled water, ethanol, methanol and acetone) were studied, and their results compared using Pearson’s Correlation. The plant extracts were also subjected to RP-HPLC for detection and quantitation of naturally occurring phenolic compounds using six phenolic standards (Gallic acid, Vanillin, Catechol, Ferrulic acid, p-coumaric acid and Caffeic acid). The highest total phenolic content (7.22 mg/g GAE) and FRAP value (64.98 mg/g TE) were observed in aqueous dry root extract. The acetone extract of fresh leaf (57.08 mg/g of RE) was found richest in total flavonoids, while the methanolic extract of fresh fruit uniquely exhibited strong free radical scavenging activity as evidenced by the low IC50 value (34.37 mg/ml) in DPPH assay. The RP-HPLC analysis revealed that Catechol and Gallic acid were most abundantly found phenolic compounds in extracts of H. isora L. The total phenolic content showed strong positive correlation with free radical scavenging activity (FRAP and DPPH assays) in both fresh and dry plant parts, suggesting that phenols are the main compounds responsible for the antioxidant activity. The root of H. isora L. was found rich in phenolics and antioxidant capacity indicating its strong potential for medicinal use, followed by fruit, leaf and bark.},
 year = {2014}
}

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