Evaluation of Skin Protective Effect Using <i>in Vitro</i> Cell Line Models of Biofield Energy-Consciousness Energy Healing Treated Herbomineral Formula

William Dean Plikerd; Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; Mayank Gangwar; Snehasis Jana

doi:doi:10.11648/j.ajhr.20170503.13

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Evaluation of Skin Protective Effect Using in Vitro Cell Line Models of Biofield Energy-Consciousness Energy Healing Treated Herbomineral Formula

William Dean Plikerd, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana

Published in American Journal of Health Research (Volume 5, Issue 3)

Received: 28 March 2017 Accepted: 10 April 2017 Published: 9 May 2017

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Abstract

Alternative or natural systems of medicine are practiced worldwide that mostly emphasize research of herbal formulations, which might be helpful against various disorders. The objective of this study was to evaluate the influence of The Trivedi Effect^®-Consciousness Energy Healing Treatment on an herbomineral test formulation and cell medium (DMEM) for the skin health. The formulation contained minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two equal parts. One part received Biofield Energy Treatment (BT) by William Dean Plikerd and other part was denoted as the untreated test items (UT). MTT assay showed that the test formulation was found safe and nontoxic with greater than 75% cell viability against various tested concentrations. The cell proliferation using BrdU method showed improved cell proliferation by 43.63% and 10.97% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation group, respectively at 17.5 µg/mL compared with the untreated group. The collagen level was significantly increased by 2.65% and 23.56% at 2.5 and 1.25 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The elastin level was increased by 8.34% at a concentrations of 2.5 µg/mL in the BT-DMEM + BT-Test formulation group compared with the untreated group. However, the hyaluronic acid (HA) level was increased by 2.60% at 0.625 µg/mL in the BT-DMEM + UT-Test formulation group compared with the untreated group. Moreover, melanin synthesis was decreased by 14.22% and 17.61% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at a concentration of 0.125 µg/mL. Anti-wrinkling activity in HFF-1 cells showed improve cell viability by 14.54% and 11.32% at 1.25 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively compared with the untreated group. Wound healing scratch assay results showed significantly increased healing rates by 10.5% and 8.3% in the HFF-1 and HaCaT cells lines, respectively in the Biofield Energy Healing based test formulation. In conclusion, The Trivedi Effect^® based test formulation and DMEM could be useful to improve the skin ECM components, along with protection against UV-B rays, which could be useful for the development of an effective cosmetic product for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles and/or change in the skin color, etc. as an anti-ageing, skin-whitening, and anti-wrinkling.

Published in	American Journal of Health Research (Volume 5, Issue 3)
DOI	10.11648/j.ajhr.20170503.13
Page(s)	65-75
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Consciousness Energy Healing Treatment, B16-F10, HaCaT, HFF-1, Hyaluronic Acid, Scratch Assay, Tetrahydrocurcumin

References

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APA Style

William Dean Plikerd, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. (2017). Evaluation of Skin Protective Effect Using in Vitro Cell Line Models of Biofield Energy-Consciousness Energy Healing Treated Herbomineral Formula. American Journal of Health Research, 5(3), 65-75. https://doi.org/10.11648/j.ajhr.20170503.13

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ACS Style

William Dean Plikerd; Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak, et al. Evaluation of Skin Protective Effect Using in Vitro Cell Line Models of Biofield Energy-Consciousness Energy Healing Treated Herbomineral Formula. Am. J. Health Res. 2017, 5(3), 65-75. doi: 10.11648/j.ajhr.20170503.13

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AMA Style

William Dean Plikerd, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. Evaluation of Skin Protective Effect Using in Vitro Cell Line Models of Biofield Energy-Consciousness Energy Healing Treated Herbomineral Formula. Am J Health Res. 2017;5(3):65-75. doi: 10.11648/j.ajhr.20170503.13

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@article{10.11648/j.ajhr.20170503.13,
  author = {William Dean Plikerd and Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and Mayank Gangwar and Snehasis Jana},
  title = {Evaluation of Skin Protective Effect Using in Vitro Cell Line Models of Biofield Energy-Consciousness Energy Healing Treated Herbomineral Formula},
  journal = {American Journal of Health Research},
  volume = {5},
  number = {3},
  pages = {65-75},
  doi = {10.11648/j.ajhr.20170503.13},
  url = {https://doi.org/10.11648/j.ajhr.20170503.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajhr.20170503.13},
  abstract = {Alternative or natural systems of medicine are practiced worldwide that mostly emphasize research of herbal formulations, which might be helpful against various disorders. The objective of this study was to evaluate the influence of The Trivedi Effect®-Consciousness Energy Healing Treatment on an herbomineral test formulation and cell medium (DMEM) for the skin health. The formulation contained minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two equal parts. One part received Biofield Energy Treatment (BT) by William Dean Plikerd and other part was denoted as the untreated test items (UT). MTT assay showed that the test formulation was found safe and nontoxic with greater than 75% cell viability against various tested concentrations. The cell proliferation using BrdU method showed improved cell proliferation by 43.63% and 10.97% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation group, respectively at 17.5 µg/mL compared with the untreated group. The collagen level was significantly increased by 2.65% and 23.56% at 2.5 and 1.25 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The elastin level was increased by 8.34% at a concentrations of 2.5 µg/mL in the BT-DMEM + BT-Test formulation group compared with the untreated group. However, the hyaluronic acid (HA) level was increased by 2.60% at 0.625 µg/mL in the BT-DMEM + UT-Test formulation group compared with the untreated group. Moreover, melanin synthesis was decreased by 14.22% and 17.61% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at a concentration of 0.125 µg/mL. Anti-wrinkling activity in HFF-1 cells showed improve cell viability by 14.54% and 11.32% at 1.25 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively compared with the untreated group. Wound healing scratch assay results showed significantly increased healing rates by 10.5% and 8.3% in the HFF-1 and HaCaT cells lines, respectively in the Biofield Energy Healing based test formulation. In conclusion, The Trivedi Effect® based test formulation and DMEM could be useful to improve the skin ECM components, along with protection against UV-B rays, which could be useful for the development of an effective cosmetic product for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles and/or change in the skin color, etc. as an anti-ageing, skin-whitening, and anti-wrinkling.},
 year = {2017}
}

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TY  - JOUR
T1  - Evaluation of Skin Protective Effect Using in Vitro Cell Line Models of Biofield Energy-Consciousness Energy Healing Treated Herbomineral Formula
AU  - William Dean Plikerd
AU  - Mahendra Kumar Trivedi
AU  - Alice Branton
AU  - Dahryn Trivedi
AU  - Gopal Nayak
AU  - Mayank Gangwar
AU  - Snehasis Jana
Y1  - 2017/05/09
PY  - 2017
N1  - https://doi.org/10.11648/j.ajhr.20170503.13
DO  - 10.11648/j.ajhr.20170503.13
T2  - American Journal of Health Research
JF  - American Journal of Health Research
JO  - American Journal of Health Research
SP  - 65
EP  - 75
PB  - Science Publishing Group
SN  - 2330-8796
UR  - https://doi.org/10.11648/j.ajhr.20170503.13
AB  - Alternative or natural systems of medicine are practiced worldwide that mostly emphasize research of herbal formulations, which might be helpful against various disorders. The objective of this study was to evaluate the influence of The Trivedi Effect®-Consciousness Energy Healing Treatment on an herbomineral test formulation and cell medium (DMEM) for the skin health. The formulation contained minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two equal parts. One part received Biofield Energy Treatment (BT) by William Dean Plikerd and other part was denoted as the untreated test items (UT). MTT assay showed that the test formulation was found safe and nontoxic with greater than 75% cell viability against various tested concentrations. The cell proliferation using BrdU method showed improved cell proliferation by 43.63% and 10.97% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation group, respectively at 17.5 µg/mL compared with the untreated group. The collagen level was significantly increased by 2.65% and 23.56% at 2.5 and 1.25 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The elastin level was increased by 8.34% at a concentrations of 2.5 µg/mL in the BT-DMEM + BT-Test formulation group compared with the untreated group. However, the hyaluronic acid (HA) level was increased by 2.60% at 0.625 µg/mL in the BT-DMEM + UT-Test formulation group compared with the untreated group. Moreover, melanin synthesis was decreased by 14.22% and 17.61% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at a concentration of 0.125 µg/mL. Anti-wrinkling activity in HFF-1 cells showed improve cell viability by 14.54% and 11.32% at 1.25 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively compared with the untreated group. Wound healing scratch assay results showed significantly increased healing rates by 10.5% and 8.3% in the HFF-1 and HaCaT cells lines, respectively in the Biofield Energy Healing based test formulation. In conclusion, The Trivedi Effect® based test formulation and DMEM could be useful to improve the skin ECM components, along with protection against UV-B rays, which could be useful for the development of an effective cosmetic product for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles and/or change in the skin color, etc. as an anti-ageing, skin-whitening, and anti-wrinkling.
VL  - 5
IS  - 3
ER  -

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Author Information

William Dean Plikerd

Trivedi Global, Inc., Henderson, NV, USA
Mahendra Kumar Trivedi

Trivedi Global, Inc., Henderson, NV, USA
Alice Branton

Trivedi Global, Inc., Henderson, NV, USA
Dahryn Trivedi

Trivedi Global, Inc., Henderson, NV, USA
Gopal Nayak

Trivedi Global, Inc., Henderson, NV, USA
Mayank Gangwar

Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana

Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

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APA Style

William Dean Plikerd, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. (2017). Evaluation of Skin Protective Effect Using in Vitro Cell Line Models of Biofield Energy-Consciousness Energy Healing Treated Herbomineral Formula. American Journal of Health Research, 5(3), 65-75. https://doi.org/10.11648/j.ajhr.20170503.13

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ACS Style

William Dean Plikerd; Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak, et al. Evaluation of Skin Protective Effect Using in Vitro Cell Line Models of Biofield Energy-Consciousness Energy Healing Treated Herbomineral Formula. Am. J. Health Res. 2017, 5(3), 65-75. doi: 10.11648/j.ajhr.20170503.13

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AMA Style

William Dean Plikerd, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. Evaluation of Skin Protective Effect Using in Vitro Cell Line Models of Biofield Energy-Consciousness Energy Healing Treated Herbomineral Formula. Am J Health Res. 2017;5(3):65-75. doi: 10.11648/j.ajhr.20170503.13

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@article{10.11648/j.ajhr.20170503.13,
  author = {William Dean Plikerd and Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and Mayank Gangwar and Snehasis Jana},
  title = {Evaluation of Skin Protective Effect Using in Vitro Cell Line Models of Biofield Energy-Consciousness Energy Healing Treated Herbomineral Formula},
  journal = {American Journal of Health Research},
  volume = {5},
  number = {3},
  pages = {65-75},
  doi = {10.11648/j.ajhr.20170503.13},
  url = {https://doi.org/10.11648/j.ajhr.20170503.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajhr.20170503.13},
  abstract = {Alternative or natural systems of medicine are practiced worldwide that mostly emphasize research of herbal formulations, which might be helpful against various disorders. The objective of this study was to evaluate the influence of The Trivedi Effect®-Consciousness Energy Healing Treatment on an herbomineral test formulation and cell medium (DMEM) for the skin health. The formulation contained minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two equal parts. One part received Biofield Energy Treatment (BT) by William Dean Plikerd and other part was denoted as the untreated test items (UT). MTT assay showed that the test formulation was found safe and nontoxic with greater than 75% cell viability against various tested concentrations. The cell proliferation using BrdU method showed improved cell proliferation by 43.63% and 10.97% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation group, respectively at 17.5 µg/mL compared with the untreated group. The collagen level was significantly increased by 2.65% and 23.56% at 2.5 and 1.25 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The elastin level was increased by 8.34% at a concentrations of 2.5 µg/mL in the BT-DMEM + BT-Test formulation group compared with the untreated group. However, the hyaluronic acid (HA) level was increased by 2.60% at 0.625 µg/mL in the BT-DMEM + UT-Test formulation group compared with the untreated group. Moreover, melanin synthesis was decreased by 14.22% and 17.61% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at a concentration of 0.125 µg/mL. Anti-wrinkling activity in HFF-1 cells showed improve cell viability by 14.54% and 11.32% at 1.25 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively compared with the untreated group. Wound healing scratch assay results showed significantly increased healing rates by 10.5% and 8.3% in the HFF-1 and HaCaT cells lines, respectively in the Biofield Energy Healing based test formulation. In conclusion, The Trivedi Effect® based test formulation and DMEM could be useful to improve the skin ECM components, along with protection against UV-B rays, which could be useful for the development of an effective cosmetic product for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles and/or change in the skin color, etc. as an anti-ageing, skin-whitening, and anti-wrinkling.},
 year = {2017}
}

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TY  - JOUR
T1  - Evaluation of Skin Protective Effect Using in Vitro Cell Line Models of Biofield Energy-Consciousness Energy Healing Treated Herbomineral Formula
AU  - William Dean Plikerd
AU  - Mahendra Kumar Trivedi
AU  - Alice Branton
AU  - Dahryn Trivedi
AU  - Gopal Nayak
AU  - Mayank Gangwar
AU  - Snehasis Jana
Y1  - 2017/05/09
PY  - 2017
N1  - https://doi.org/10.11648/j.ajhr.20170503.13
DO  - 10.11648/j.ajhr.20170503.13
T2  - American Journal of Health Research
JF  - American Journal of Health Research
JO  - American Journal of Health Research
SP  - 65
EP  - 75
PB  - Science Publishing Group
SN  - 2330-8796
UR  - https://doi.org/10.11648/j.ajhr.20170503.13
AB  - Alternative or natural systems of medicine are practiced worldwide that mostly emphasize research of herbal formulations, which might be helpful against various disorders. The objective of this study was to evaluate the influence of The Trivedi Effect®-Consciousness Energy Healing Treatment on an herbomineral test formulation and cell medium (DMEM) for the skin health. The formulation contained minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two equal parts. One part received Biofield Energy Treatment (BT) by William Dean Plikerd and other part was denoted as the untreated test items (UT). MTT assay showed that the test formulation was found safe and nontoxic with greater than 75% cell viability against various tested concentrations. The cell proliferation using BrdU method showed improved cell proliferation by 43.63% and 10.97% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation group, respectively at 17.5 µg/mL compared with the untreated group. The collagen level was significantly increased by 2.65% and 23.56% at 2.5 and 1.25 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The elastin level was increased by 8.34% at a concentrations of 2.5 µg/mL in the BT-DMEM + BT-Test formulation group compared with the untreated group. However, the hyaluronic acid (HA) level was increased by 2.60% at 0.625 µg/mL in the BT-DMEM + UT-Test formulation group compared with the untreated group. Moreover, melanin synthesis was decreased by 14.22% and 17.61% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at a concentration of 0.125 µg/mL. Anti-wrinkling activity in HFF-1 cells showed improve cell viability by 14.54% and 11.32% at 1.25 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively compared with the untreated group. Wound healing scratch assay results showed significantly increased healing rates by 10.5% and 8.3% in the HFF-1 and HaCaT cells lines, respectively in the Biofield Energy Healing based test formulation. In conclusion, The Trivedi Effect® based test formulation and DMEM could be useful to improve the skin ECM components, along with protection against UV-B rays, which could be useful for the development of an effective cosmetic product for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles and/or change in the skin color, etc. as an anti-ageing, skin-whitening, and anti-wrinkling.
VL  - 5
IS  - 3
ER  -

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