Carbohydrate Catabolism in Adult <i>Onchocerca volvulus:</i> An Immunohistochemical Study

Seidu Mahmood Abdulai; Adams Abdul Rashid; Gyasi Kwame Richard; Tettey Yao; Adunyame Lois; Nkansah Obenewaa Dinah; Wiredu Kwame Edwin

doi:doi:10.11648/j.ajbls.20160403.13

Carbohydrate Catabolism in Adult Onchocerca volvulus: An Immunohistochemical Study

Seidu Mahmood Abdulai, Adams Abdul Rashid, Gyasi Kwame Richard, Tettey Yao, Adunyame Lois, Nkansah Obenewaa Dinah, Wiredu Kwame Edwin

Published in American Journal of Biomedical and Life Sciences (Volume 4, Issue 3)

Received: 14 April 2016 Accepted: 25 April 2016 Published: 11 May 2016

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Abstract

Onchocerca volvulus is a parasite responsible for Onchocerciasis whose main pathology is blindness. Existing treatment and control approaches are not entirely successful, with some, fraught with safety challenges. Due to these problems, the need for developing safer and effective drugs to combat the disease has become imperative. However O. volvulus materials are restricted by ethical concerns due to its strict human preference. To overcome these concerns some researchers use animal models of closely related species to obtain biological information on O. volvulus and drugs developed from these sources of information have so far failed to kill the adult O. volvulus. Realistic targets for drug development against O. volvulus could be detected directly in O. volvulus rather than its closely related species. We performed immunohistochemical detection of three major enzymes (G6PD, LDH and PDHK2) involved in carbohydrate metabolism on paraffin processed archival O. volvulus nodules. We observed that up to 64.5% of worms in the paraffin processed nodules had detectable LDH, 61.1% had G6PD and 56.7% had PDHK2 and that most of the enzymes were stored in the muscles of the adult worm. These observations suggest that the adult O. volvulus can operate the glycolytic, Pentose and Entner-Douhoroff pathways either independently or concurrently suggesting that any drug aimed at preventing the adult worm from utilising carbohydrates must target all three enzymes.

Published in	American Journal of Biomedical and Life Sciences (Volume 4, Issue 3)
DOI	10.11648/j.ajbls.20160403.13
Page(s)	35-40
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Antibodies, Antigens, Carbohydrate catabolism, Onchocerciasis, Nodules, Enzymes

References

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APA Style

Seidu Mahmood Abdulai, Adams Abdul Rashid, Gyasi Kwame Richard, Tettey Yao, Adunyame Lois, et al. (2016). Carbohydrate Catabolism in Adult Onchocerca volvulus: An Immunohistochemical Study. American Journal of Biomedical and Life Sciences, 4(3), 35-40. https://doi.org/10.11648/j.ajbls.20160403.13

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ACS Style

Seidu Mahmood Abdulai; Adams Abdul Rashid; Gyasi Kwame Richard; Tettey Yao; Adunyame Lois, et al. Carbohydrate Catabolism in Adult Onchocerca volvulus: An Immunohistochemical Study. Am. J. Biomed. Life Sci. 2016, 4(3), 35-40. doi: 10.11648/j.ajbls.20160403.13

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AMA Style

Seidu Mahmood Abdulai, Adams Abdul Rashid, Gyasi Kwame Richard, Tettey Yao, Adunyame Lois, et al. Carbohydrate Catabolism in Adult Onchocerca volvulus: An Immunohistochemical Study. Am J Biomed Life Sci. 2016;4(3):35-40. doi: 10.11648/j.ajbls.20160403.13

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@article{10.11648/j.ajbls.20160403.13,
  author = {Seidu Mahmood Abdulai and Adams Abdul Rashid and Gyasi Kwame Richard and Tettey Yao and Adunyame Lois and Nkansah Obenewaa Dinah and Wiredu Kwame Edwin},
  title = {Carbohydrate Catabolism in Adult Onchocerca volvulus: An Immunohistochemical Study},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {4},
  number = {3},
  pages = {35-40},
  doi = {10.11648/j.ajbls.20160403.13},
  url = {https://doi.org/10.11648/j.ajbls.20160403.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20160403.13},
  abstract = {Onchocerca volvulus is a parasite responsible for Onchocerciasis whose main pathology is blindness. Existing treatment and control approaches are not entirely successful, with some, fraught with safety challenges. Due to these problems, the need for developing safer and effective drugs to combat the disease has become imperative. However O. volvulus materials are restricted by ethical concerns due to its strict human preference. To overcome these concerns some researchers use animal models of closely related species to obtain biological information on O. volvulus and drugs developed from these sources of information have so far failed to kill the adult O. volvulus. Realistic targets for drug development against O. volvulus could be detected directly in O. volvulus rather than its closely related species. We performed immunohistochemical detection of three major enzymes (G6PD, LDH and PDHK2) involved in carbohydrate metabolism on paraffin processed archival O. volvulus nodules. We observed that up to 64.5% of worms in the paraffin processed nodules had detectable LDH, 61.1% had G6PD and 56.7% had PDHK2 and that most of the enzymes were stored in the muscles of the adult worm. These observations suggest that the adult O. volvulus can operate the glycolytic, Pentose and Entner-Douhoroff pathways either independently or concurrently suggesting that any drug aimed at preventing the adult worm from utilising carbohydrates must target all three enzymes.},
 year = {2016}
}

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TY - JOUR
T1 - Carbohydrate Catabolism in Adult Onchocerca volvulus: An Immunohistochemical Study
AU - Seidu Mahmood Abdulai
AU - Adams Abdul Rashid
AU - Gyasi Kwame Richard
AU - Tettey Yao
AU - Adunyame Lois
AU - Nkansah Obenewaa Dinah
AU - Wiredu Kwame Edwin
Y1 - 2016/05/11
PY - 2016
N1 - https://doi.org/10.11648/j.ajbls.20160403.13
DO - 10.11648/j.ajbls.20160403.13
T2 - American Journal of Biomedical and Life Sciences
JF - American Journal of Biomedical and Life Sciences
JO - American Journal of Biomedical and Life Sciences
SP - 35
EP - 40
PB - Science Publishing Group
SN - 2330-880X
UR - https://doi.org/10.11648/j.ajbls.20160403.13
AB - Onchocerca volvulus is a parasite responsible for Onchocerciasis whose main pathology is blindness. Existing treatment and control approaches are not entirely successful, with some, fraught with safety challenges. Due to these problems, the need for developing safer and effective drugs to combat the disease has become imperative. However O. volvulus materials are restricted by ethical concerns due to its strict human preference. To overcome these concerns some researchers use animal models of closely related species to obtain biological information on O. volvulus and drugs developed from these sources of information have so far failed to kill the adult O. volvulus. Realistic targets for drug development against O. volvulus could be detected directly in O. volvulus rather than its closely related species. We performed immunohistochemical detection of three major enzymes (G6PD, LDH and PDHK2) involved in carbohydrate metabolism on paraffin processed archival O. volvulus nodules. We observed that up to 64.5% of worms in the paraffin processed nodules had detectable LDH, 61.1% had G6PD and 56.7% had PDHK2 and that most of the enzymes were stored in the muscles of the adult worm. These observations suggest that the adult O. volvulus can operate the glycolytic, Pentose and Entner-Douhoroff pathways either independently or concurrently suggesting that any drug aimed at preventing the adult worm from utilising carbohydrates must target all three enzymes.
VL - 4
IS - 3
ER -

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Author Information

Seidu Mahmood Abdulai

Department of Medical laboratory science, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana
Adams Abdul Rashid

Department of Medical laboratory science, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana
Gyasi Kwame Richard

Department of Pathology, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana
Tettey Yao

Department of Pathology, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana
Adunyame Lois

Department of Medical laboratory science, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana
Nkansah Obenewaa Dinah

Department of Medical laboratory science, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana
Wiredu Kwame Edwin

Department of Medical laboratory science, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana

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APA Style

Seidu Mahmood Abdulai, Adams Abdul Rashid, Gyasi Kwame Richard, Tettey Yao, Adunyame Lois, et al. (2016). Carbohydrate Catabolism in Adult Onchocerca volvulus: An Immunohistochemical Study. American Journal of Biomedical and Life Sciences, 4(3), 35-40. https://doi.org/10.11648/j.ajbls.20160403.13

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ACS Style

Seidu Mahmood Abdulai; Adams Abdul Rashid; Gyasi Kwame Richard; Tettey Yao; Adunyame Lois, et al. Carbohydrate Catabolism in Adult Onchocerca volvulus: An Immunohistochemical Study. Am. J. Biomed. Life Sci. 2016, 4(3), 35-40. doi: 10.11648/j.ajbls.20160403.13

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AMA Style

Seidu Mahmood Abdulai, Adams Abdul Rashid, Gyasi Kwame Richard, Tettey Yao, Adunyame Lois, et al. Carbohydrate Catabolism in Adult Onchocerca volvulus: An Immunohistochemical Study. Am J Biomed Life Sci. 2016;4(3):35-40. doi: 10.11648/j.ajbls.20160403.13

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@article{10.11648/j.ajbls.20160403.13,
  author = {Seidu Mahmood Abdulai and Adams Abdul Rashid and Gyasi Kwame Richard and Tettey Yao and Adunyame Lois and Nkansah Obenewaa Dinah and Wiredu Kwame Edwin},
  title = {Carbohydrate Catabolism in Adult Onchocerca volvulus: An Immunohistochemical Study},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {4},
  number = {3},
  pages = {35-40},
  doi = {10.11648/j.ajbls.20160403.13},
  url = {https://doi.org/10.11648/j.ajbls.20160403.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20160403.13},
  abstract = {Onchocerca volvulus is a parasite responsible for Onchocerciasis whose main pathology is blindness. Existing treatment and control approaches are not entirely successful, with some, fraught with safety challenges. Due to these problems, the need for developing safer and effective drugs to combat the disease has become imperative. However O. volvulus materials are restricted by ethical concerns due to its strict human preference. To overcome these concerns some researchers use animal models of closely related species to obtain biological information on O. volvulus and drugs developed from these sources of information have so far failed to kill the adult O. volvulus. Realistic targets for drug development against O. volvulus could be detected directly in O. volvulus rather than its closely related species. We performed immunohistochemical detection of three major enzymes (G6PD, LDH and PDHK2) involved in carbohydrate metabolism on paraffin processed archival O. volvulus nodules. We observed that up to 64.5% of worms in the paraffin processed nodules had detectable LDH, 61.1% had G6PD and 56.7% had PDHK2 and that most of the enzymes were stored in the muscles of the adult worm. These observations suggest that the adult O. volvulus can operate the glycolytic, Pentose and Entner-Douhoroff pathways either independently or concurrently suggesting that any drug aimed at preventing the adult worm from utilising carbohydrates must target all three enzymes.},
 year = {2016}
}

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